Department of Epidemiology, State Key Laboratory of Oncogene and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Epidemiology, State Key Laboratory of Oncogene and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; School of Public Health, Fudan University, Shanghai, China.
J Nutr. 2023 Sep;153(9):2709-2716. doi: 10.1016/j.tjnut.2023.07.011. Epub 2023 Jul 27.
Previous findings about the influence of dietary intakes of the branched-chain amino acid (BCAA) on their plasma concentrations have been limited and inconsistent, and evidence from the Chinese population was lacking.
This study aimed to investigate the diet-plasma BCAA correlations in Chinese male and female adults.
This cross-sectional study was based on a nested case-control study within 2 prospective population-based cohorts in Shanghai, China. Diet information was collected by the food frequency questionnaires. Plasma BCAA concentrations were measured by ultraperformance liquid chromatography coupled with tandem mass spectrometry. Spearman correlations and linear regression models were conducted to examine the relationships between dietary BCAA intakes and plasma BCAA. The multivariable model was adjusted for age at the interview, total energy intake, time of blood collection from last meal, dietary patterns, body mass index (in kg/m), type 2 diabetes, and physical activity.
A total of 322 males (median age of 57.0 y) and 187 females (median age of 60.0 y) were included in this cross-sectional study. The geometric means of dietary intake of leucine, isoleucine, valine, and BCAA were 4937.7, 3029.6, 3268.5, and 11237.4 mg/d in males, and 4125.7, 2567.8, 2754.3, and 9449.4 mg/d in females. The geometric means of plasma concentrations of leucine, isoleucine, valine, and BCAA were 181.9, 65.0, 219.8, and 469.4 μM/L in males and 161.6, 61.1, 206.5, and 431.6 μM/L in females. Only leucine (r = 0.1660, P = 0.0028) and total BCAA (r = 0.1348, P = 0.0155) in males exhibited weak positive correlation coefficients. After adjustment for the covariates, leucine, isoleucine, valine, and total BCAA in dietary intakes and plasma were not correlated in both males and females.
In Chinese male and female adults, dietary intakes are not major determinants of plasma concentrations of BCAA, and plasma concentrations might not be reflected by usual dietary intakes of BCAA.
以往关于支链氨基酸（BCAA）饮食摄入量对其血浆浓度影响的研究结果有限且不一致，且缺乏来自中国人群的证据。
本研究旨在调查中国成年男性和女性的饮食-血浆 BCAA 相关性。
本横断面研究基于中国上海 2 个前瞻性人群队列内的巢式病例对照研究。通过食物频率问卷收集饮食信息。采用超高效液相色谱-串联质谱法测定血浆 BCAA 浓度。采用 Spearman 相关和线性回归模型检验膳食 BCAA 摄入量与血浆 BCAA 之间的关系。多变量模型调整了访谈时的年龄、总能量摄入、上次进餐至采血的时间、膳食模式、体重指数（kg/m²）、2 型糖尿病和体力活动。
本横断面研究共纳入 322 名男性（中位年龄 57.0 岁）和 187 名女性（中位年龄 60.0 岁）。男性膳食中亮氨酸、异亮氨酸、缬氨酸和 BCAA 的摄入量几何均数分别为 4937.7、3029.6、3268.5 和 11237.4mg/d，女性分别为 4125.7、2567.8、2754.3 和 9449.4mg/d。男性血浆亮氨酸、异亮氨酸、缬氨酸和 BCAA 的浓度几何均数分别为 181.9、65.0、219.8 和 469.4μM/L，女性分别为 161.6、61.1、206.5 和 431.6μM/L。仅男性的亮氨酸（r=0.1660，P=0.0028）和总 BCAA（r=0.1348，P=0.0155）呈弱正相关。在调整了协变量后，男性和女性膳食中的亮氨酸、异亮氨酸、缬氨酸和总 BCAA 摄入量与血浆中的浓度均无相关性。
在中国成年男性和女性中，膳食摄入量不是 BCAA 血浆浓度的主要决定因素，BCAA 的血浆浓度可能不能反映其通常的膳食摄入量。
