Xie Guangrong, Zhang Qipeng, Fang Zhengfeng, Che Lianqiang, Lin Yan, Xu Shengyu, Zhuo Yong, Hua Lun, Jiang Xuemei, Li Jian, Sun Mengmeng, Zou Yuanfeng, Huang Chao, Li Lixia, Wu De, Feng Bin
Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
College of Science, Sichuan Agricultural University, Ya'an 625014, China.
Antioxidants (Basel). 2023 Jun 28;12(7):1355. doi: 10.3390/antiox12071355.
Dietary oxidized fat contains harmful materials such as hydrogen peroxide and malondialdehyde (MDA). Excessive oxidized fat intake during pregnancy and lactation not only leads to maternal body injury but also damages offspring health. Our previous study demonstrated that vitamin D (VD) had antioxidative capability in sows. This study was conducted to investigate the effect of maternal VD and inulin supplementation in oxidized oil diet on the growth performance and oxidative stress of their offspring. Sixty 5-month-old C57BL/6N female mice were randomly divided into five groups: Control group (basal diet, n = 12), OF group (oxidized-soybean-oil-replaced diet, n = 12), OFV group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD, n = 12), OFI group (oxidized-soybean-oil-replaced diet + 5% inulin, n = 12) and OFVI group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD + 5% inulin, n = 12). Mice were fed with the respective diet during pregnancy and lactation. The offspring were then slaughtered on day 21 of age at weaning. Results showed that a maternal oxidized oil diet impaired body weight and liver weight gain of offspring during lactation compared to the control group, while maternal VD, inulin or VD and inulin mixture supplementation reversed this effect. In addition, the activity of T-AOC in the liver of offspring was lower in the OF group than that in the control group, but could be restored by maternal VD and inulin mixture supplementation. Furthermore, the gene expression of both proinflammatory and anti-inflammatory cytokines, such as , and , in offspring liver were downregulated by a maternal oxidized oil diet compared with the control group, but they were restored by maternal VD or VD and inulin mixture supplementation. The expressions of and were decreased by a maternal oxidized oil diet compared with the control group, while they could be increased by VD or VD and inulin mixture supplementation. Conclusion: maternal oxidized oil diet intake could impair the growth performance by inducing oxidative stress, but this can be relieved by maternal VD and inulin supplementation.
膳食氧化脂肪含有过氧化氢和丙二醛（MDA）等有害物质。孕期和哺乳期过量摄入氧化脂肪不仅会导致母体身体损伤，还会损害后代健康。我们之前的研究表明，维生素D（VD）对母猪具有抗氧化能力。本研究旨在探讨母体补充VD和菊粉对氧化油日粮后代生长性能和氧化应激的影响。将60只5月龄的C57BL/6N雌性小鼠随机分为五组：对照组（基础日粮，n = 12）、OF组（氧化大豆油替代日粮，n = 12）、OFV组（氧化大豆油替代日粮 + 7000 IU/kg VD，n = 12）、OFI组（氧化大豆油替代日粮 + 5%菊粉，n = 12）和OFVI组（氧化大豆油替代日粮 + 7000 IU/kg VD + 5%菊粉，n = 12）。小鼠在怀孕和哺乳期饲喂相应日粮。后代在断奶后第21天屠宰。结果表明，与对照组相比，母体氧化油日粮会损害后代哺乳期的体重和肝脏增重，而母体补充VD、菊粉或VD与菊粉混合物可逆转这种影响。此外，OF组后代肝脏中总抗氧化能力（T-AOC）的活性低于对照组，但母体补充VD和菊粉混合物可使其恢复。此外，与对照组相比，母体氧化油日粮会下调后代肝脏中促炎和抗炎细胞因子（如 、 和 ）的基因表达，但母体补充VD或VD与菊粉混合物可使其恢复。与对照组相比，母体氧化油日粮会降低 和 的表达，而VD或VD与菊粉混合物补充可使其增加。结论：母体摄入氧化油日粮可通过诱导氧化应激损害生长性能，但母体补充VD和菊粉可缓解这种情况。
