Institute of Animal Nutrition, Northeast Agricultural University, Harbin, Heilongjiang 150030, People's Republic of China.
J Agric Food Chem. 2022 May 25;70(20):6224-6238. doi: 10.1021/acs.jafc.2c01100. Epub 2022 May 12.
As a food contaminant, oxidized oil or lipid oxidative products have been proven to exert toxicological effects on the growth and development of animals and humans. Research shows that maternal oxidative stress damage might transmit to another generation by epigenetic modulation. However, current evidence is still not clear on the mechanism of the effects of dietary oxidized oil during pregnancy on the two generations. This study employed a rat model fed with oxidized soybean oil (OSO) during pregnancy and lactation to explore the effects of the oxidative degree (0, 200, 400, and 800 mequiv of O/kg) on the placental RNA methylation and DNA methylation in offspring jejunum. The results showed that following the ingestion of OSO, the placental genes of different mA methylation were significantly enriched to nutrient metabolic processes and hormone activity. In addition, the intestine in offspring hypofunctioned observably, such as reducing the height of villi and the level of anti-inflammatory cytokine. Furthermore, maternal intake of OSO during pregnancy can damage the intestinal barrier function of offspring by inhibiting the proliferation and differentiation of intestinal epithelial cells and reducing the activity of intestinal DNA methyltransferase. In conclusion, this study reinforces the assertion that maternal OSO consumption during gestation and lactation negatively affects the placental health and intestinal development of suckling pups.
作为一种食物污染物，氧化油或脂质氧化产物已被证明对动物和人类的生长发育具有毒理学效应。研究表明，母体氧化应激损伤可能通过表观遗传调节传递给下一代。然而，目前关于孕期饮食中氧化油对两代人影响的机制仍不清楚。本研究采用妊娠和哺乳期给予氧化大豆油（OSO）的大鼠模型，探讨氧化程度（0、200、400 和 800 mequiv O/kg）对后代空肠胎盘 RNA 甲基化和 DNA 甲基化的影响。结果表明，摄入 OSO 后，不同 mA 甲基化的胎盘基因明显富集到营养代谢过程和激素活性。此外，后代的肠道功能明显减弱，如绒毛高度降低和抗炎细胞因子水平降低。此外，母体在妊娠期间摄入 OSO 可通过抑制肠上皮细胞的增殖和分化以及降低肠道 DNA 甲基转移酶的活性，损害后代的肠道屏障功能。总之，本研究进一步证实，母体在妊娠和哺乳期摄入 OSO 会对哺乳期幼崽的胎盘健康和肠道发育产生负面影响。
