Sen Nisa Beril, Guzelmeric Etil, Vovk Irena, Glavnik Vesna, Kırmızıbekmez Hasan, Yesilada Erdem
Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Kayisdagi Cad., Atasehir, Istanbul 34755, Türkiye.
Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia.
Antioxidants (Basel). 2023 Jul 6;12(7):1394. doi: 10.3390/antiox12071394.
Bee pollen, known as a 'life-giving dust', is a product of honeybees using flower pollen grains and combining them with their saliva secretions. Thus, flower pollen could be an indicator of the bee pollen botanical source. Identification of bee pollen sources is a highly crucial process for the evaluation of its health benefits, as chemical composition is directly related to its pharmacological activity. In this study, the chemical profiles, contents of phenolic marker compounds and pharmacological activities of L. (ivy) bee pollen samples from Türkiye and Slovenia, as well as ivy flower pollen grains, were compared. High-performance thin-layer chromatography (HPTLC) analyses revealed that pollen samples, regardless of where they were collected, have similar chemical profiles due to the fact that they have the same botanical origins. Marker compounds afzelin, platanoside and quercetin-3--β-glucopyranosyl-(1→2)-β-galactopyranoside, common to both bee pollen and flower pollen, were isolated from bee pollen, and their structures were elucidated by nuclear magnetic resonance (NMR) and mass spectrometry (MS). These three compounds, as well as chlorogenic acid and 3,5-dicaffeoylquinic acid (found in flower pollen), were quantified using high-performance liquid chromatography (HPLC) analyses. tests and effect-directed analyses were used to evaluate the xanthine oxidase inhibition and antioxidant activity of the marker compounds and extracts from flower pollen and bee pollen. This is the first report comparing chemical profiles and related bioactivities of the flower pollen and bee pollen of the same botanical origin, as well as the first report of the chemical profile and related bioactivities of ivy flower pollen.
蜂花粉被誉为“赋予生命的尘埃”，是蜜蜂采集花粉粒并将其与唾液分泌物混合而成的产物。因此，花粉可能是蜂花粉植物来源的一个指标。鉴定蜂花粉来源对于评估其健康益处是一个至关重要的过程，因为化学成分直接与其药理活性相关。在本研究中，对来自土耳其和斯洛文尼亚的常春藤蜂花粉样本以及常春藤花粉粒的化学特征、酚类标记化合物含量和药理活性进行了比较。高效薄层色谱（HPTLC）分析表明，无论花粉样本采集于何处，由于它们具有相同的植物来源，其化学特征相似。从蜂花粉中分离出了蜂花粉和花粉共有的标记化合物阿夫齐林、悬铃木苷和槲皮素-3--β-吡喃葡萄糖基-(1→2)-β-吡喃半乳糖苷，并通过核磁共振（NMR）和质谱（MS）对其结构进行了阐明。使用高效液相色谱（HPLC）分析对这三种化合物以及绿原酸和3,5-二咖啡酰奎宁酸（存在于花粉中）进行了定量。采用试验和效应导向分析来评估标记化合物以及花粉和蜂花粉提取物的黄嘌呤氧化酶抑制和抗氧化活性。这是第一份比较相同植物来源的花粉和蜂花粉化学特征及相关生物活性的报告，也是第一份关于常春藤花粉化学特征及相关生物活性的报告。
Zotero 插件
