Zhou Xiechen, Li Yan, Li Tao, Cao Junyang, Guan Zijian, Xu Tianlong, Jia Guiyan, Ma Gaopeng, Zhao Rui
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Animals (Basel). 2023 Jul 14;13(14):2306. doi: 10.3390/ani13142306.
Diarrhea is one of the most common causes of death in young piglets. Porcine rotavirus (PoRV) belongs to the genus within the family , and is considered to be the primary pathogen causing diarrhea in piglets. L. (POL) has been reported to alleviate diarrhea and viral infections. However, the antiviral effect of L. polysaccharide (POL-P), an active component of POL, on PoRV infection remains unclear. This study demonstrated that the safe concentration range of POL-P in IPEC-J2 cells is 0-400 μg/mL. POL-P (400 μg/mL) effectively inhibits PoRV infection in IPEC-J2 cells, reducing the expression of rotavirus VP6 protein, mRNA and virus titer. Furthermore, on the basis of viral life cycle analysis, we showed that POL-P can decrease the expression of PoRV VP6 protein, mRNA, and virus titer during the internalization and replication stages of PoRV. POL-P exerts antiviral effects by increasing IFN-α expression and decreasing the expression levels of TNF-α, IL-6, and IL-10 inflammatory factors. Overall, our study found that POL-P is a promising candidate for anti-PoRV drugs.
腹泻是仔猪最常见的死亡原因之一。猪轮状病毒（PoRV）属于 科 属，被认为是导致仔猪腹泻的主要病原体。已报道罗伊氏乳杆菌（L.）可缓解腹泻和病毒感染。然而，罗伊氏乳杆菌多糖（POL-P）作为罗伊氏乳杆菌的一种活性成分，对PoRV感染的抗病毒作用仍不清楚。本研究表明，POL-P在IPEC-J2细胞中的安全浓度范围为0-400μg/mL。POL-P（400μg/mL）可有效抑制IPEC-J2细胞中的PoRV感染，降低轮状病毒VP6蛋白、mRNA的表达及病毒滴度。此外，基于病毒生命周期分析，我们发现POL-P可在PoRV的内化和复制阶段降低PoRV VP6蛋白、mRNA的表达及病毒滴度。POL-P通过增加IFN-α表达并降低TNF-α、IL-6和IL-10炎症因子的表达水平发挥抗病毒作用。总体而言，我们的研究发现POL-P是一种很有前景的抗PoRV药物候选物。
