Liu Yueyue, Zhao Lu, Xie Yunhui, Chen Zhi, Yang Shifa, Yin Bin, Li Guiming, Guo Huijun, Lin Shuqian, Wu Jiaqiang
Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, 250023, Shandong, PR China.
Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, 250023, Shandong, PR China; College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, 271018, Shandong, PR China.
Microb Pathog. 2021 May;154:104832. doi: 10.1016/j.micpath.2021.104832. Epub 2021 Mar 26.
Porcine epidemic diarrhea virus (PEDV), especially variants, causes a highly contagious enteric disease which could give rise to huge economic losses in the swine industry worldwide. Portulaca oleracea L. has been reported to regulate intestine disease and involved in viral infections. However, the underlying mechanisms of Portulaca oleracea L. extracts against PEDV have not been fully elucidated. In this study, the antiviral effects and potential mechanisms of Portulaca oleracea L. extracts against PEDV were investigated in vitro. We first examined the inhibitory effects of different Portulaca oleracea L. extracts on the PEDV(JX-16 strain) in vitro and found that the water extract of Portulaca oleracea L.(PO)could significantly inhibit PEDV replication by 92.73% on VH cells and 63.07% on Vero cells. Furthermore, time-course analysis showed PO inhibited PEDV replication during the adsorption period of infectious cycle. Western blot and indirect immunofluorescence assay indicated that PO down-regulated the S protein expression in a dose-dependent manner. In addition, our results demonstrated the ability of PO to inhibit PEDV replication in VH cells by down-regulating the cytokine levels (TNF-α,IL-22 and IFN-α) and inhibiting the NF-κB signaling pathway activated by PEDV. Thus, Portulaca oleracea L extracts have potential utility in the preventive and therapeutic strategies for PEDV infection.
猪流行性腹泻病毒（PEDV），尤其是变异株，可引发一种高度传染性的肠道疾病，会给全球养猪业造成巨大经济损失。据报道，马齿苋可调节肠道疾病并参与病毒感染。然而，马齿苋提取物抗PEDV的潜在机制尚未完全阐明。在本研究中，对马齿苋提取物抗PEDV的体外抗病毒作用及潜在机制进行了研究。我们首先检测了不同马齿苋提取物对体外PEDV（JX - 16株）的抑制作用，发现马齿苋水提取物（PO）在VH细胞上可显著抑制PEDV复制达92.73%，在Vero细胞上为63.07%。此外，时间进程分析表明PO在感染周期的吸附期抑制PEDV复制。蛋白质免疫印迹法和间接免疫荧光试验表明PO以剂量依赖方式下调S蛋白表达。此外，我们的结果证明PO通过下调细胞因子水平（TNF-α、IL - 22和IFN-α）以及抑制PEDV激活的NF-κB信号通路来抑制VH细胞中的PEDV复制。因此，马齿苋提取物在PEDV感染的预防和治疗策略中具有潜在应用价值。
