Melbourne School of Land and Environment, The University of Melbourne, Parkville, Victoria, Australia.
Vet Microbiol. 2010 Jan 6;140(1-2):56-62. doi: 10.1016/j.vetmic.2009.07.020. Epub 2009 Aug 8.
Bovine rotavirus (BRV) has been detected in both dairy and beef cattle herds worldwide. Stool samples collected from calves in the Gippsland region of Victoria, Australia were screened to determine the presence of BRV. A total of 100 faecal samples were collected from calves with and without diarrhoea across three farms during 2004 and 2005. Group A BRV was detected in 26% of faecal samples (22 from diarrheic calves and four from asymptomatic calves). Genotyping analysis of rotavirus positive samples indicated that G6P[5] was the most prevalent genotype (38.5%) followed by G6P[5+11] (15.4%). G10P[11] and G6+G10P[5] were each detected at a rate of 7.7%, and G6+G10P[11] was found in a single sample (3.8%). Seven samples (26.9%) could not be G and/or P typed. Thirty percent of the BRV positive samples were mixed infections, indicating that individual calves were co-infected with more than one strain of rotavirus. The G6P[5] strains exhibited high VP7 identity (>97% amino acid identity) with B-60, a G6 strain identified in Victorian calves during 1988. A G10P[11] isolate was closely related (>97% amino acid identity in VP7 and VP4 proteins) to a Victorian G10P[11] strain (B-11) also identified during 1988. This study demonstrates that BRV is a contributing pathogen to diarrhoeal disease in Victorian calves, with sequence analysis suggesting long-term conservation of the VP7 protein over a 16-year period.
牛轮状病毒（BRV）已在全球的奶牛和肉牛群中被检测到。从澳大利亚维多利亚吉普斯兰地区的小牛粪便样本中筛选出 BRV。2004 年至 2005 年期间，在三个农场的腹泻和非腹泻小牛中总共收集了 100 份粪便样本。在粪便样本中检测到 A 组 BRV 占 26%（22 份来自腹泻小牛，4 份来自无症状小牛）。对轮状病毒阳性样本的基因分型分析表明，G6P[5]是最常见的基因型（38.5%），其次是 G6P[5+11]（15.4%）。G10P[11]和 G6+G10P[5]的检出率分别为 7.7%，G6+G10P[11]仅在一个样本中检出（3.8%）。有 7 个样本（26.9%）无法进行 G 和/或 P 型分型。30%的 BRV 阳性样本为混合感染，表明单个小牛同时感染了一种以上的轮状病毒株。G6P[5]株与 B-60（1988 年在维多利亚州小牛中鉴定的 G6 株）的 VP7 具有高度同源性（>97%氨基酸同一性）。G10P[11]分离株与 1988 年同时鉴定的维多利亚州 G10P[11]株（B-11）在 VP7 和 VP4 蛋白中高度同源（>97%氨基酸同一性）。本研究表明 BRV 是维多利亚州小牛腹泻病的致病病原体，序列分析表明 VP7 蛋白在 16 年内保持长期保守。
