Meletis Georgios, Malousi Andigoni, Tychala Areti, Kassomenaki Angeliki, Vlachodimou Nikoletta, Mantzana Paraskevi, Metallidis Simeon, Skoura Lemonia, Protonotariou Efthymia
Department of Microbiology, AHEPA University Hospital, School of Medicine, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece.
Laboratory of Biological Chemistry, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Antibiotics (Basel). 2023 Jul 20;12(7):1206. doi: 10.3390/antibiotics12071206.
NDM carbapenemase-encoding genes disseminate commonly among Enterobacterales through transferable plasmids carrying additional resistance determinants. Apart from the intra-species dissemination, the inter-species exchange of plasmids seems to play an additional important role in the spread of . We here present the genetics related to the isolation of three species (, , and ) harboring the gene from a single patient in Greece. Bacterial identification and antimicrobial susceptibility testing were performed using the Vitek2. Whole genome sequencing and bioinformatic tools were used to identify resistance genes and plasmids. harboring plasmids were found in all three isolates. Moreover, the plasmid constructs of the respective incomplete or circular contigs showed that the and its neighboring genes form a cluster that was found in all isolates. Our microbiological findings, together with the patient's history, suggest the in vivo transfer of the -containing cluster through three different species in a single patient.
携带额外耐药决定簇的可转移质粒使编码NDM碳青霉烯酶的基因在肠杆菌科细菌中广泛传播。除了种内传播外，质粒的种间交换似乎在……的传播中发挥了额外的重要作用。我们在此介绍与从希腊一名患者中分离出携带blaNDM基因的三种细菌（肺炎克雷伯菌、大肠埃希菌和产气肠杆菌）相关的遗传学情况。使用Vitek2进行细菌鉴定和抗菌药物敏感性测试。利用全基因组测序和生物信息学工具来鉴定耐药基因和质粒。在所有三个分离株中均发现携带质粒的细菌。此外，各自不完整或环状重叠群的质粒构建显示，blaNDM及其邻近基因形成了一个在所有分离株中均存在的簇。我们的微生物学发现，连同患者的病史，提示含blaNDM簇在一名患者体内通过三种不同细菌进行了转移。
