Umar Muhammad, Farooq Tahir, Tegg Robert S, Thangavel Tamilarasan, Wilson Calum R
New Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, 13 St. Johns Avenue, New Town, TAS 7008, Australia.
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Plants (Basel). 2022 Mar 25;11(7):884. doi: 10.3390/plants11070884.
(BrYV), a tentative species in the genus , of the family, is a phloem-restricted and aphid-transmitted virus with at least three genotypes (A, B, and C). It has been found across mainland China, South Korea, and Japan. BrYV was previously undescribed in Tasmania, and its genetic variability in the state remains unknown. Here, we describe a near-complete genome sequence of BrYV (genotype A) isolated from in Tasmania using next-generation sequencing and sanger sequencing of RT-PCR products. BrYV-Tas (GenBank Accession no. OM469309) possesses a genome of 5516 nucleotides (nt) and shares higher sequence identity (about 90%) with other BrYV isolates. Phylogenetic analyses showed variability in the clustering patterns of the individual genes of BrYV-Tas. Recombination analysis revealed beginning and ending breakpoints at nucleotide positions 1922 to 5234 nt, with the BrYV isolate LC428359 and BrYV isolate KY310572 identified as major and minor parents, respectively. Results of the evolutionary analysis showed that the majority of the codons for each gene are evolving under purifying selection, though a few codons were also detected to have positive selection pressure. Taken together, our findings will facilitate an understanding of the evolutionary dynamics and genetic diversity of BrYV.
芸薹黄化病毒（BrYV）是黄症病毒科一个暂定种，是一种局限于韧皮部且由蚜虫传播的病毒，至少有三个基因型（A、B和C）。该病毒已在中国大陆、韩国和日本被发现。此前在塔斯马尼亚尚未发现BrYV，其在该州的遗传变异性仍不清楚。在此，我们使用下一代测序技术和RT-PCR产物的桑格测序法，描述了从塔斯马尼亚的[具体植物名称]分离出的BrYV（基因型A）的近乎完整的基因组序列。BrYV-Tas（GenBank登录号：OM469309）拥有一个5516个核苷酸（nt）的基因组，与其他BrYV分离株具有较高的序列同一性（约90%）。系统发育分析显示BrYV-Tas各个基因的聚类模式存在变异性。重组分析揭示了核苷酸位置1922至5234 nt处的起始和终止断点，分别将BrYV分离株LC428359和BrYV分离株KY310572鉴定为主要亲本和次要亲本。进化分析结果表明，每个基因的大多数密码子在纯化选择下进化，不过也检测到少数密码子具有正选择压力。综上所述，我们的研究结果将有助于了解BrYV的进化动态和遗传多样性。
