Arun Arunachalam, Shanthi Annaiyan, Raveendran Muthurajan, Seenivasan Nagachandrabose, Pushpam Ramamoorthy, Shandeep Ganeshan
Department of Nematology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India.
Directorate of Research, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India.
Biology (Basel). 2023 Jul 11;12(7):987. doi: 10.3390/biology12070987.
Rice ( L.) is one of the most widely grown crops in the world, and is a staple food for more than half of the global total population. Root-knot nematodes (RKNs), spp., and especially , seem to be significant rice pests, which makes them the most economically important plant-parasitic nematode in this crop. RKNs develop a feeding site in galls by causing host cells to differentiate into hypertrophied, multinucleate, metabolically active cells known as giant cells. This grazing framework gives the nematode a constant food source, permitting it to develop into a fecund female and complete its life cycle inside the host root. effector proteins involved in nematode parasitism, including pioneer genes, were functionally characterized in earlier studies. Molecular modelling and docking studies were performed on protein targets, such as β-1,4-endoglucanase, pectate lyase, phospholipase B-like protein, and G protein-coupled receptor kinase, to understand the binding affinity of Beta-D-Galacturonic Acid, 2,6,10,15,19,23-hexamethyltetracosane, (2S)-2-amino-3-phenylpropanoic acid, and 4-O-Beta-D-Galactopyranosyl-Alpha-D-Glucopyranose against ligand molecules of rice. This study discovered important molecular aspects of plant-nematode interaction and candidate effector proteins that were regulated by -infected rice plants. To the best of our knowledge, this is the first study to describe 's molecular adaptation to host parasitism.
稻(Oryza L.)是世界上种植最广泛的作物之一,是全球一半以上人口的主食。根结线虫(RKNs),Meloidogyne spp.,尤其是南方根结线虫(Meloidogyne incognita),似乎是重要的水稻害虫,这使其成为该作物中经济上最重要的植物寄生线虫。根结线虫通过使宿主细胞分化为肥大的、多核的、代谢活跃的细胞(即巨型细胞)在虫瘿中形成取食位点。这种取食结构为线虫提供了持续的食物来源,使其能够发育成繁殖力强的雌虫并在宿主根内完成其生命周期。早期研究对线虫寄生相关的效应蛋白,包括先驱基因,进行了功能表征。对β-1,4-内切葡聚糖酶、果胶酸裂解酶、磷脂酶B样蛋白和G蛋白偶联受体激酶等蛋白质靶点进行了分子建模和对接研究,以了解β-D-半乳糖醛酸、2,6,10,15,19,23-六甲基二十四烷、(2S)-2-氨基-3-苯丙酸和4-O-β-D-吡喃半乳糖基-α-D-吡喃葡萄糖对水稻配体分子的结合亲和力。本研究发现了植物-线虫相互作用的重要分子方面以及受南方根结线虫感染的水稻植株调控的候选效应蛋白。据我们所知,这是第一项描述南方根结线虫对宿主寄生的分子适应性的研究。