Morgado Micaela C, Sousa Mónica, Marques Cláudia, Coelho André B, Costa Júlio A, Seabra André
Research Centre in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal.
Portugal Football School, Portuguese Football Federation (FPF), 1495-433 Cruz Quebrada, Portugal.
Children (Basel). 2023 Jul 19;10(7):1242. doi: 10.3390/children10071242.
Childhood obesity continues to represent a growing challenge, and it has been associated with gut microbiota dysbiosis. This study examines the gut microbiota composition in overweight and obese school children and assesses whether a 12-week multidisciplinary intervention can induce changes in the gut microbiota. The intervention, which combined recreational football and nutritional education, was implemented among 15 school children, aged 7-10 years, with a Body Mass Index ≥ 85th percentile. The children were assigned into two groups: Football Group ( = 9) and Nutrition and Football Group ( = 6). Faecal samples were collected at the beginning and end of the program and analysed by sequencing the 16S rRNA gene. Over the intervention, a significant decrease was found collectively for Bifidobacterium genera ( = 0.011) and for genera in the Football Group ( = 0.021). The relative abundance of ( = 0.002) and ( = 0.009) was negatively correlated with moderate to vigorous physical activity (MVPA), while was positively correlated with MVPA ( = 0.010) and with the daily intake of protein ( = 0.008). Our findings suggest that a multidisciplinary intervention was capable of inducing limited but significant positive changes in the gut microbiota composition in overweight and obese school children.
儿童肥胖仍然是一个日益严峻的挑战，并且它与肠道微生物群失调有关。本研究调查了超重和肥胖学童的肠道微生物群组成，并评估了为期12周的多学科干预是否能引起肠道微生物群的变化。该干预措施结合了休闲足球和营养教育，在15名年龄在7至10岁、体重指数≥第85百分位数的学童中实施。这些儿童被分为两组：足球组（n = 9）和营养与足球组（n = 6）。在项目开始和结束时收集粪便样本，并通过对16S rRNA基因进行测序来分析。在干预过程中，发现双歧杆菌属总体上显著减少（P = 0.011），足球组中的某属也显著减少（P = 0.021）。某菌属（P = 0.002）和另一菌属（P = 0.009）的相对丰度与中度至剧烈身体活动（MVPA）呈负相关，而另一菌属与MVPA呈正相关（P = 0.010），并与蛋白质的每日摄入量呈正相关（P = 0.008）。我们的研究结果表明，多学科干预能够在超重和肥胖学童的肠道微生物群组成中引起有限但显著的积极变化。
