Plath Andre M Souza, Huber Stephanie, Alfarano Serena R, Abbott Daniel F, Hu Minghan, Mougel Victor, Isa Lucio, Ferguson Stephen J
Laboratory for Orthopaedic Technology, ETH Zurich, 8092 Zurich, Switzerland.
Laboratory of Food and Soft Materials, ETH Zurich, 8092 Zurich, Switzerland.
Bioengineering (Basel). 2023 Jun 27;10(7):771. doi: 10.3390/bioengineering10070771.
Osteoarthritis scaffold-based grafts fail because of poor integration with the surrounding soft tissue and inadequate tribological properties. To circumvent this, we propose electrospun poly(ε-caprolactone)/zein-based scaffolds owing to their biomimetic capabilities. The scaffold surfaces were characterized using Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, static water contact angles, and profilometry. Scaffold biocompatibility properties were assessed by measuring protein adsorption (Bicinchoninic Acid Assay), cell spreading (stained F-actin), and metabolic activity (PrestoBlue™ Cell Viability Reagent) of primary bovine chondrocytes. The data show that zein surface segregation in the membranes not only completely changed the hydrophobic behavior of the materials, but also increased the cell yield and metabolic activity on the scaffolds. The surface segregation is verified by the infrared peak at 1658 cm, along with the presence and increase in N1 content in the survey XPS. This observation could explain the decrease in the water contact angles from 125° to approximately 60° in zein-comprised materials and the decrease in the protein adsorption of both bovine serum albumin and synovial fluid by half. Surface nano roughness in the PCL/zein samples additionally benefited the radial spreading of bovine chondrocytes. This study showed that co-electrospun PCL/zein scaffolds have promising surface and biocompatibility properties for use in articular-tissue-engineering applications.
基于支架的骨关节炎移植失败是因为与周围软组织的整合性差以及摩擦学性能不足。为了规避这一问题，我们提出使用电纺聚（ε-己内酯）/玉米醇溶蛋白基支架，因为它们具有仿生能力。使用傅里叶变换红外光谱、X射线光电子能谱、静态水接触角和轮廓仪对支架表面进行了表征。通过测量原代牛软骨细胞的蛋白质吸附（二辛可宁酸测定法）、细胞铺展（F-肌动蛋白染色）和代谢活性（PrestoBlue™细胞活力试剂）来评估支架的生物相容性。数据表明，膜中玉米醇溶蛋白的表面偏析不仅完全改变了材料的疏水行为，还提高了支架上的细胞产量和代谢活性。通过1658 cm处的红外峰以及XPS全谱中N1含量的存在和增加来验证表面偏析。这一观察结果可以解释含玉米醇溶蛋白材料的水接触角从125°降至约60°，以及牛血清白蛋白和滑液的蛋白质吸附减少一半的现象。PCL/玉米醇溶蛋白样品中的表面纳米粗糙度还有利于牛软骨细胞的径向铺展。这项研究表明，共电纺PCL/玉米醇溶蛋白支架在关节组织工程应用中具有良好的表面和生物相容性。
