Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, 07122 Palma de Mallorca, Spain.
Biomolecules. 2023 Jun 29;13(7):1061. doi: 10.3390/biom13071061.
Pathological calcifications may consist of calcium oxalate (CaOx), hydroxyapatite (HAP), and brushite (BRU). The objective of this study was to evaluate the effect of phytate (inositol hexakisphosphate, InsP6), InsP6 hydrolysates, and individual lower InsPs (InsP5, InsP4, InsP3, and InsP2) on the crystallization of CaOx, HAP and BRU in artificial urine. All of the lower InsPs seem to inhibit the crystallization of calcium salts in biological fluids, although our in vitro results showed that InsP6 and InsP5 were stronger inhibitors of CaOx crystallization, and InsP5 and InsP4 were stronger inhibitors of BRU crystallization. For the specific in vitro experimental conditions we examined, the InsPs had very weak effects on HAP crystallization, although it is likely that a different mechanism is responsible for HAP crystallization in vivo. For example, calciprotein particles seem to have an important role in the formation of cardiovascular calcifications in vivo. The experimental conditions that we examined partially reproduced the in vivo conditions of CaOx and BRU crystallization, but not the in vivo conditions of HAP crystallization.
病理性钙化可由草酸钙 (CaOx)、羟磷灰石 (HAP) 和磷酸氢钙 (BRU) 组成。本研究旨在评估植酸（肌醇六磷酸，InsP6）、InsP6 水解产物和个别低 InsP（InsP5、InsP4、InsP3 和 InsP2）对人工尿液中 CaOx、HAP 和 BRU 结晶的影响。所有低 InsP 似乎都能抑制生物液中钙盐的结晶，尽管我们的体外结果表明 InsP6 和 InsP5 是 CaOx 结晶的更强抑制剂，InsP5 和 InsP4 是 BRU 结晶的更强抑制剂。对于我们检查的特定体外实验条件，InsPs 对 HAP 结晶的影响非常弱，尽管体内 HAP 结晶可能涉及不同的机制。例如，钙结合蛋白颗粒在体内心血管钙化的形成中似乎起着重要作用。我们检查的实验条件部分再现了体内 CaOx 和 BRU 结晶的条件，但未再现体内 HAP 结晶的条件。
