Grases F, Costa-Bauzá A, Berga F, Rodríguez A, Gomila R M, Martorell G, Martínez-Cignoni M R
Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, Ctra Valldemossa, km 7.5, 07122 Palma de Mallorca, Spain.
Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, Ctra Valldemossa, km 7.5, 07122 Palma de Mallorca, Spain.
Life Sci. 2018 Jan 1;192:33-37. doi: 10.1016/j.lfs.2017.11.023. Epub 2017 Nov 16.
Previous studies demonstrated a remarkable increase of urinary InsP by topical administration. However, the methodology used for InsP analysis was not specific. The aim of this paper is to measure urinary inositol phosphates InsPs using more advanced methodologies and to compare the results with those obtained by the non-specific method.
We fed 12 female rats with a diet without InsP for 16days. Then, we administered a topical InsP gel at high doses for 7days (50mgInsP/day) or at low doses for 28days (20mgInsP/day). We measured urine levels InsPs using a nonspecific method (based on the ability of InsPs to complex Al) and levels of InsP by a specific method (using polyacrylamide gel electrophoresis). Identification of different InsPs was performed by MS.
At baseline, after dietary deprivation of InsP, rats only excreted InsP in their urine, and there was no detectable InsP or other InsPs. Rats given the high dose treatment for 7days had abundant urinary InsP, but also had other InsPs in their urine; cessation of InsP administration led to decreased levels of urinary InsPs. Rats given the low dose treatment for 28days had increasing levels of urinary InsPs over time. The maximum urinary InsP was at 21days, after which InsPs excretion decreased.
We conclude that the skin can absorb InsP from a topical gel, and that InsP is excreted in the urine, along with other InsPs (InsP, InsP, InsP, and InsP).
先前的研究表明，局部给药后尿中肌醇磷酸（InsP）显著增加。然而，用于InsP分析的方法并不特异。本文的目的是使用更先进的方法测量尿中肌醇磷酸（InsPs），并将结果与非特异方法所得结果进行比较。
我们给12只雌性大鼠喂食不含InsP的饲料16天。然后，我们以高剂量（50mg InsP/天）局部给予InsP凝胶7天，或以低剂量（20mg InsP/天）给予28天。我们使用非特异方法（基于InsPs与铝络合的能力）测量尿中InsPs水平，并使用特异方法（聚丙烯酰胺凝胶电泳）测量InsP水平。通过质谱对不同的InsPs进行鉴定。
在基线时，即饮食中缺乏InsP后，大鼠尿中仅排泄InsP，未检测到其他InsP或InsPs。接受高剂量治疗7天的大鼠尿中InsP丰富，但也有其他InsPs；停止给予InsP导致尿中InsPs水平下降。接受低剂量治疗28天的大鼠尿中InsPs水平随时间增加。尿中InsP最高值出现在第21天，之后InsPs排泄减少。
我们得出结论，皮肤可从局部凝胶中吸收InsP，且InsP与其他InsPs（InsP、InsP、InsP和InsP）一起经尿液排泄。
