Yu Chong, Shan Jingyu, Ju Hao, Chen Xiao, Xu Guangsen, Wu Yanchao
School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, China.
Foods. 2023 Jul 13;12(14):2692. doi: 10.3390/foods12142692.
This work presents the fabrication of ternary nanoparticles (Z/S/C NPs) comprising zein (Z), soy protein isolate (SPI) and carboxymethylcellulose sodium (CMC-Na) through a pH-driven method. The results showed that the smallest particle size (71.41 nm) and the most stable zeta potential, measuring -49.97 mV, were achieved with the following ratio of ternary nanoparticles Z/SPI/CMC-Na (2:3:3). The surface morphology of the nanoparticles was further analyzed using transmission electron microscopy, and the synthesized nanoparticles were utilized to encapsulate curcumin (Cur), a hydrophobic, bioactive compound. The nanoparticles were characterized using a particle size analyzer, infrared spectroscopy, and X-ray diffraction (XRD) techniques. The results revealed that the formation of nanoparticles and the encapsulation of Cur were driven by electrostatic, hydrogen-bonding and hydrophobic interactions. The drug loading efficiency (EE%) of Z/S/C-cur nanoparticles reached 90.90%. The Z/S/C ternary nanoparticles demonstrated enhanced storage stability, photostability and simulated the gastrointestinal digestion of Cur. The release of Cur and variations in the particle size of nanoparticles were investigated across different stages of digestion. The biocompatibility of the Z/S/C ternary nanoparticles was assessed by conducting cell viability assays on HepG2 and L-O2 cells, which showed no signs of cytotoxicity. These results suggested that the ternary composite nanoparticles have potential in delivering nutritional foods and health-promoting bioactive substances.
本研究通过pH驱动法制备了包含玉米醇溶蛋白（Z）、大豆分离蛋白（SPI）和羧甲基纤维素钠（CMC-Na）的三元纳米颗粒（Z/S/C NPs）。结果表明，三元纳米颗粒Z/SPI/CMC-Na（2:3:3）具有最小粒径（71.41 nm）和最稳定的ζ电位，为-49.97 mV。使用透射电子显微镜进一步分析了纳米颗粒的表面形态，并将合成的纳米颗粒用于包封姜黄素（Cur），一种疏水性生物活性化合物。使用粒度分析仪、红外光谱和X射线衍射（XRD）技术对纳米颗粒进行了表征。结果表明，纳米颗粒的形成以及Cur的包封是由静电、氢键和疏水相互作用驱动的。Z/S/C-姜黄素纳米颗粒的载药效率（EE%）达到90.90%。Z/S/C三元纳米颗粒表现出增强的储存稳定性、光稳定性，并模拟了Cur的胃肠道消化过程。研究了不同消化阶段Cur的释放情况以及纳米颗粒粒径的变化。通过对HepG2和L-O2细胞进行细胞活力测定来评估Z/S/C三元纳米颗粒的生物相容性，结果显示没有细胞毒性迹象。这些结果表明，三元复合纳米颗粒在递送营养食品和促进健康的生物活性物质方面具有潜力。
