Weihai Marine Organism & Medical Technology Research Institute, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150006, PR China.
Weihai Marine Organism & Medical Technology Research Institute, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150006, PR China.
Colloids Surf B Biointerfaces. 2022 Sep;217:112685. doi: 10.1016/j.colsurfb.2022.112685. Epub 2022 Jul 1.
In the last few decades, zein has been extensively studied owing to its wide commercial availability and the ability to self-assemble into nanosphere structure to encapsulate biologically active substances for targeted delivery. This work emphasized on comparing the encapsulation efficiency of hydrophobic active biomolecules and the physicochemical stability of composite nanoparticles (NPs) made up of zein- and foxtail millet prolamin (FP) -caseinate. Puerarin, resveratrol, diosmetin, and curcumin with various LogP values were selected as model drugs to study the single/co-encapsulation capacity, storage stability, and in vitro release profiles. Both LogP values (polarity) and specific structure are the main factors affecting the encapsulation efficiency. FP-based NPs could entrap more resveratrol, which may be related to the lower hydrophobic amino acid content of FP in comparison with that of zein. Co-encapsulation, in vitro release and long-term storage stability experiments confirmed that the model drugs were encapsulated in different NP regions mediated by polarity. Moreover, co-encapsulation changed the environment of curcumin from relatively polar microenvironment to hydrophobic regions. These hydrophobic regions retained significantly more curcumin during long-term storage stability. Overall, our results suggest that the hydrophobic amino acid composition of prolamin affects the encapsulation capacity. Various bioactives were encapsulated in the prolamin-based NPs via polarity mediation, and co-encapsulation could effectively retain the active molecules during storage.
在过去的几十年中，由于玉米醇溶蛋白广泛的商业可用性和自组装成纳米球结构以封装生物活性物质用于靶向递送的能力，因此对其进行了广泛的研究。这项工作强调了比较疏水性活性生物分子的包封效率和由玉米醇溶蛋白-和谷子醇溶蛋白-酪蛋白组成的复合纳米颗粒（NPs）的物理化学稳定性。选择具有不同 LogP 值的葛根素、白藜芦醇、橙皮苷和姜黄素作为模型药物，研究其单一/共包封能力、储存稳定性和体外释放特性。LogP 值（极性）和特定结构是影响包封效率的主要因素。基于 FP 的 NPs 可以包封更多的白藜芦醇，这可能与 FP 中的疏水性氨基酸含量低于玉米醇溶蛋白有关。共包封、体外释放和长期储存稳定性实验证实，模型药物通过极性被包封在不同的 NP 区域中。此外，共包封将姜黄素的环境从相对极性的微环境转变为疏水区。在长期储存稳定性期间，这些疏水区保留了更多的姜黄素。总的来说，我们的结果表明，醇溶蛋白中的疏水性氨基酸组成影响包封能力。通过极性介导，各种生物活性物质被包封在醇溶蛋白基 NPs 中，共包封可以在储存过程中有效保留活性分子。
