André Rebeca, Pacheco Rita, Alves Ana Catarina, Santos Hugo M, Bourbon Mafalda, Serralheiro Maria Luísa
BioISI-Biosystems & Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.
Department of Chemical Engineering, ISEL-Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emídio Navarro, 1, 1959-007 Lisboa, Portugal.
Foods. 2023 Jul 20;12(14):2758. doi: 10.3390/foods12142758.
A brown seaweed consumed worldwide, , has been used to prevent atherosclerosis and hypercholesterolemia, among other uses. However, the mechanisms of action that lead to these effects are not yet fully understood. This work aims to study the in vitro effect of an aqueous extract of , previously characterized as rich in phlorotannins and peptides, on the expression of different proteins involved in the synthesis and transport of cholesterol. A proteomic analysis, Western blot, and qRT-PCR analysis were performed to identify protein changes in HepG2 cells exposed to 0.25 mg/mL of the extract for 24 h. The proteomic results demonstrated that, in liver cells, the extract decreases the expression of four proteins involved in the cholesterol biosynthesis process (CYP51A1, DHCR24, HMGCS1 and HSD17B7). Additionally, a 12.76% and 18.40% decrease in the expression of two important transporters proteins of cholesterol, NPC1L1 and ABCG5, respectively, was also observed, as well as a 30% decrease in NPC1L1 mRNA levels in the cells exposed to the extract compared to control cells. Our study reveals some of the mechanisms underlying the actions of bioactive compounds from that may explain its previously reported hypocholesterolemic effect, future prospecting its use as a functional food.
一种在全球范围内被食用的褐藻，已被用于预防动脉粥样硬化和高胆固醇血症等。然而，导致这些效果的作用机制尚未完全明确。这项工作旨在研究一种先前被鉴定为富含间苯三酚鞣质和肽的褐藻水提取物对参与胆固醇合成和运输的不同蛋白质表达的体外影响。进行了蛋白质组学分析、蛋白质印迹法和定量逆转录聚合酶链反应分析，以确定暴露于0.25 mg/mL褐藻提取物24小时的HepG2细胞中的蛋白质变化。蛋白质组学结果表明，在肝细胞中，该提取物降低了参与胆固醇生物合成过程的四种蛋白质（CYP51A1、DHCR24、HMGCS1和HSD17B7）的表达。此外，还观察到两种重要的胆固醇转运蛋白NPC1L1和ABCG5的表达分别下降了12.76%和18.40%，并且与对照细胞相比，暴露于提取物的细胞中NPC1L1 mRNA水平下降了30%。我们的研究揭示了褐藻生物活性化合物作用的一些潜在机制，这可能解释了其先前报道的降胆固醇作用，为其作为功能性食品的未来应用提供了前景。
