CBIOS-Research Center for Biosciences & Health Technologies, Universidade Lusófona, Campo Grande 376, 1749-024 Lisboa, Portugal.
Department of Chemical Engineering, ISEL-Instituto Superior de Engenharia de Lisboa, Rua Conselheiro Emídio Navarro, 1, 1959-007 Lisboa, Portugal.
Mar Drugs. 2024 Apr 20;22(4):187. doi: 10.3390/md22040187.
High blood cholesterol levels are a major risk factor for cardiovascular diseases. A purified aqueous extract of , rich in phlorotannins and peptides, has been described for its potential to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells' metabolites and proteins was analysed to gain a deeper understanding of its mode of action on lipids' metabolism, particularly concerning the absorption and transport of exogenous cholesterol. Caco-2 cells, differentiated into enterocytes, were exposed to the extract, and analysed by untargeted metabolomics and proteomics. The results of the metabolomic analysis showed statistically significant differences in glutathione content of cells exposed to the extract compared to control cells, along with an increased expression of fatty acid amides in exposed cells. A proteomic analysis showed an increased expression in cells exposed to the extract compared to control cells of FAB1 and NPC1, proteins known to be involved in lipid metabolism and transport. To the extent of our knowledge, this study is the first use of untargeted metabolomics and a proteomic analysis to investigate the effects of on differentiated Caco-2 cells, offering insights into the molecular mechanism of the extract's compounds on intestinal cells.
血液中胆固醇水平偏高是引发心血管疾病的主要风险因素之一。一种富含原花青素和肽的纯化水提物，因其抑制胆固醇生物合成和肠道吸收的潜力而备受关注。在这项工作中，分析了该提取物对肠道细胞代谢物和蛋白质的影响，以更深入地了解其在脂质代谢方面的作用机制，特别是涉及外源性胆固醇的吸收和转运。将分化为肠细胞的 Caco-2 细胞暴露于提取物中，并通过非靶向代谢组学和蛋白质组学进行分析。代谢组学分析的结果表明，与对照细胞相比，暴露于提取物的细胞中的谷胱甘肽含量存在统计学上的显著差异，同时暴露细胞中的脂肪酸酰胺表达增加。蛋白质组学分析表明，与对照细胞相比，暴露于提取物的细胞中 FAB1 和 NPC1 蛋白的表达增加，这些蛋白已知参与脂质代谢和转运。据我们所知，这项研究首次使用非靶向代谢组学和蛋白质组学分析来研究对分化的 Caco-2 细胞的影响，为提取物化合物对肠道细胞的分子机制提供了新的见解。
