Biochemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Genes (Basel). 2023 Jun 27;14(7):1349. doi: 10.3390/genes14071349.
Cigarette smoking (CS) is a major cause of various serious diseases due to tobacco chemicals. There is evidence suggesting that CS has been linked with the DNA damage repair system, as it can affect genomic stability, inducing genetic changes in the genes involved in the repair system, specifically the nucleotide excision repair (NER) pathway, affecting the function and/or regulation of these genes. Single nucleotide polymorphism (SNP), along with CS, can affect the work of the NER pathway and, therefore, could lead to different diseases. This study explored the association of four SNPs in both and genes with CS in the Saudi population. The Taq Man genotyping assay was used for 220 healthy non-smokers (control) and 201 healthy smokers to evaluate four SNPs in the gene named rs10817938, rs1800975, rs3176751, and rs3176752 and four SNPs in the gene called rs1870134, rs2228000, rs2228001, and rs2607775. In the gene, SNP rs3176751 showed a high-risk association with CS-induced diseases with all clinical parameters, including CS duration, CS intensity, gender, and age of smokers. On the other hand, SNP rs1800975 showed a statistically significant low-risk association with all clinical parameters. In addition, rs10817938 showed a high-risk association only with long-term smokers and a low-risk association only with younger smokers. A low-risk association was found in SNP rs3176752 with older smokers. In the gene, SNP rs2228001 showed a low-risk association only with female smokers. SNP rs2607775 revealed a statistically significant low-risk association with CS-induced diseases, concerning all parameters, except for male smokers. However, SNP rs2228000 and rs1870134 showed no association with CS. Overall, the study results demonstrated possible significant associations (effector/and protector) between CS and SNPs polymorphisms in DNA repair genes, such as and except for rs2228000 and rs1870134 polymorphisms.
吸烟（CS）是由于烟草化学物质导致各种严重疾病的主要原因。有证据表明，CS 与 DNA 损伤修复系统有关，因为它会影响基因组稳定性，诱导参与修复系统的基因发生遗传变化，特别是核苷酸切除修复（NER）途径，影响这些基因的功能和/或调节。单核苷酸多态性（SNP）与 CS 一起，可能会影响 NER 途径的工作，因此可能导致不同的疾病。本研究探讨了沙特人群中 和 基因中四个 SNPs 与 CS 的关联。使用 Taq Man 基因分型检测法对 220 名健康不吸烟者（对照组）和 201 名健康吸烟者进行评估，以评估 基因中命名为 rs10817938、rs1800975、rs3176751 和 rs3176752 的四个 SNPs 和 基因中命名为 rs1870134、rs2228000、rs2228001 和 rs2607775 的四个 SNPs。在 基因中，SNP rs3176751 与所有临床参数（包括 CS 持续时间、CS 强度、性别和吸烟者年龄）相关的 CS 诱导疾病具有高风险关联。另一方面，SNP rs1800975 与所有临床参数具有统计学显著的低风险关联。此外，rs10817938 仅与长期吸烟者具有高风险关联，仅与年轻吸烟者具有低风险关联。SNP rs3176752 与老年吸烟者存在低风险关联。在 基因中，SNP rs2228001 仅与女性吸烟者具有低风险关联。SNP rs2607775 与 CS 诱导的疾病具有统计学显著的低风险关联，除了男性吸烟者的所有参数外。然而，SNP rs2228000 和 rs1870134 与 CS 无关。总体而言，研究结果表明，在 DNA 修复基因（如 和 ）中，CS 与 SNP 多态性之间可能存在显著关联（效应物/和保护物），但 rs2228000 和 rs1870134 多态性除外。
