Department of Horticulture, Henan Agricultural University, Zhengzhou 450002, China.
Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Genes (Basel). 2023 Jul 7;14(7):1409. doi: 10.3390/genes14071409.
Salt and osmotic stress seriously restrict the growth, development, and productivity of horticultural crops in the greenhouse. The papain-like cysteine proteases (PLCPs) participate in multi-stress responses in plants. We previously demonstrated that salt and osmotic stress affect cysteine protease 15 of pepper ( L.) (); however, the role of in salt and osmotic stress responses is unknown. Here, the function of in regulating pepper salt and osmotic stress resistance was explored. Pepper plants were subjected to abiotic (sodium chloride, mannitol, salicylic acid, ethrel, methyl jasmonate, etc.) and biotic stress ( inoculation). The was silenced through the virus-induced gene silencing (VIGS) and transiently overexpressed in pepper plants. The full-length fragment is 1568 bp, with an open reading frame of 1032 bp, encoding a 343 amino acid protein. CaCP15 is a senescence-associated gene 12 (SAG12) subfamily member containing two highly conserved domains, Inhibitor 129 and Peptidase_C1. expression was the highest in the stems of pepper plants. The expression was induced by salicylic acid, ethrel, methyl jasmonate, and was infected by inoculation. Furthermore, was upregulated under salt and osmotic stress, and silencing in pepper enhanced salt and mannitol stress resistance. Conversely, transient overexpression of increased the sensitivity to salt and osmotic stress by reducing the antioxidant enzyme activities and negatively regulating the stress-related genes. This study indicates that negatively regulates salt and osmotic stress resistance in pepper via the ROS-scavenging.
盐和渗透胁迫严重限制了温室园艺作物的生长、发育和生产力。木瓜样半胱氨酸蛋白酶（PLCPs）参与植物的多种胁迫反应。我们之前证明盐和渗透胁迫影响辣椒（L.）中的半胱氨酸蛋白酶 15（）；然而， 的作用尚不清楚。在这里，探讨了在调节辣椒耐盐和渗透胁迫中的功能。通过非生物胁迫（氯化钠、甘露醇、水杨酸、乙烯利、茉莉酸甲酯等）和生物胁迫（接种）处理辣椒植株。通过病毒诱导的基因沉默（VIGS）沉默 ，并在辣椒植株中瞬时过表达。全长 片段长 1568bp，开放阅读框长 1032bp，编码 343 个氨基酸的蛋白质。CaCP15 是衰老相关基因 12（SAG12）亚家族成员，包含两个高度保守的结构域，抑制剂 129 和肽酶_C1。在辣椒植株的茎中表达量最高。表达受水杨酸、乙烯利、茉莉酸甲酯诱导，由 接种感染。此外，在盐和渗透胁迫下表达上调，在辣椒中沉默增强了对盐和甘露醇胁迫的抗性。相反，通过降低抗氧化酶活性和负调控应激相关基因，瞬时过表达 增加了对盐和渗透胁迫的敏感性。本研究表明，通过清除 ROS，负调控辣椒的耐盐和耐渗透胁迫。
