Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agricultural and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502324, India.
Genes (Basel). 2023 Jul 14;14(7):1447. doi: 10.3390/genes14071447.
Peanut () is an important oilseed and cash crop worldwide, contributing an important source of edible oil and protein for human nutrition. However, the incidence of stem rot disease caused by poses a major challenge to peanut cultivation, resulting in significant yield losses. In this study, a panel of 202 peanut accessions was evaluated for their resistance to stem rot by inoculating plants in the field with -infested oat grains in three environments. The mean disease index value of each environment for accessions in subsp. and subsp. showed no significant difference. Accessions from southern China displayed the lowest disease index value compared to those from other ecological regions. We used whole-genome resequencing to analyze the genotypes of the accessions and to identify significant SNPs associated with stem rot resistance through genome-wide association study (GWAS). A total of 121 significant SNPs associated with stem rot resistance in peanut were identified, with phenotypic variation explained (PVE) ranging from 12.23% to 15.51%. A total of 27 candidate genes within 100 kb upstream and downstream of 23 significant SNPs were annotated, which have functions related to recognition, signal transduction, and defense response. These significant SNPs and candidate genes provide valuable information for further validation and molecular breeding to improve stem rot resistance in peanut.
花生是一种重要的油料和经济作物，为人类营养提供了重要的食用油和蛋白质来源。然而，由 引起的茎腐病的发病率对花生种植构成了重大挑战，导致产量显著下降。在这项研究中，通过在三个环境中用感染的燕麦粒接种植物，对 202 份花生种质资源进行了茎腐病抗性评估。亚种和亚种的每个环境的平均病情指数值没有显著差异。与其他生态区的种质资源相比，来自中国南方的种质资源表现出最低的病情指数值。我们使用全基因组重测序来分析种质资源的基因型，并通过全基因组关联研究（GWAS）鉴定与茎腐病抗性相关的显著 SNP。共鉴定出与花生茎腐病抗性相关的 121 个显著 SNP，表型变异解释率（PVE）范围为 12.23%至 15.51%。在 23 个显著 SNP 的上下游 100kb 范围内共注释了 27 个候选基因，这些基因具有与识别、信号转导和防御反应相关的功能。这些显著 SNP 和候选基因为进一步验证和分子育种提供了有价值的信息，以提高花生的茎腐病抗性。
