Wang Juan, Yan Caixia, Shi Dachuan, Zhao Xiaobo, Yuan Cuiling, Sun Quanxi, Mou Yifei, Chen Haoning, Li Yuan, Li Chunjuan, Shan Shihua
Shandong Peanut Research Institute, Qingdao 266100, China.
Qingdao Academy of Agricultural Sciences, Qingdao 266100, China.
Plants (Basel). 2021 May 25;10(6):1058. doi: 10.3390/plants10061058.
Peanut ( L.) is an important oilseed crop worldwide, and peanut height has been shown to be closely related to yield, therefore a better understanding of the genetic base of plant height-related traits may allow us to have better control of crop yield. Plant height-related traits are quantitative traits that are genetically controlled by many genes, and distinct quantitive trait loci (QTLs) may be identified for different peanut accessions/genotypes. In the present study, in order to gain a more complete picture of the genetic base for peanut height-related traits, we first make use of the high quality NGS sequence data for 159 peanut accessions that are available within our research groups, to carry out a GWAS study for searching plant height-related regions. We then perform a literature survey and collect QTLs for two plant height-related traits (Ph: peanut main stem height, and Fbl: the first branch length) from earlier related QTL/GWAS studies in peanut. In total, we find 74 and 21 genomic regions that are, associated with traits Ph and Fbl, respectively. Annotation of these regions found a total of 692 and 229 genes for, respectively, Ph and Fbl, and among those genes, 158 genes are shared. KEGG and GO enrichment analyses of those candidate genes reveal that Ph- and Fbl-associated genes are both enriched in the biosynthesis of secondary metabolites, some basic processes, pathways, or complexes that are supposed to be crucial for plant development and growth.
花生（Arachis hypogaea L.）是全球重要的油料作物，花生株高已被证明与产量密切相关，因此，更好地了解株高相关性状的遗传基础可能有助于我们更好地控制作物产量。株高相关性状是由许多基因遗传控制的数量性状，不同花生种质/基因型可能鉴定出不同的数量性状位点（QTL）。在本研究中，为了更全面地了解花生株高相关性状的遗传基础，我们首先利用研究组内现有的159份花生种质的高质量NGS序列数据，进行全基因组关联研究（GWAS）以寻找株高相关区域。然后，我们进行文献调查，并从早期花生相关的QTL/GWAS研究中收集两个株高相关性状（Ph：花生主茎高度，Fbl：第一分枝长度）的QTL。我们总共分别发现了74个和21个与性状Ph和Fbl相关的基因组区域。对这些区域的注释分别发现了总共692个和229个与Ph和Fbl相关的基因，其中有158个基因是共享的。对这些候选基因的KEGG和GO富集分析表明，与Ph和Fbl相关的基因均富集于次生代谢物的生物合成、一些对植物发育和生长至关重要的基本过程、途径或复合体中。
