Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, 55365, Republic of Korea.
Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Cell Stress Chaperones. 2017 Nov;22(6):787-797. doi: 10.1007/s12192-017-0809-6. Epub 2017 Jun 20.
White Pekin duck is an important meat resource in the livestock industries. However, the temperature increase due to global warming has become a serious environmental factor in duck production, because of hyperthermia. Therefore, identifying the gene regulations and understanding the molecular mechanism for adaptation to the warmer environment will provide insightful information on the acclimation system of ducks. This study examined transcriptomic responses to heat stress treatments (3 and 6 h at 35 °C) and control (C, 25 °C) using RNA-sequencing analysis of genes from the breast muscle tissue. Based on three distinct differentially expressed gene (DEG) sets (3H/C, 6H/C, and 6H/3H), the expression patterns of significant DEGs (absolute log2 > 1.0 and false discovery rate < 0.05) were clustered into three responsive gene groups divided into upregulated and downregulated genes. Next, we analyzed the clusters that showed relatively higher expression levels in 3H/C and lower levels in 6H/C with much lower or opposite levels in 6H/3H; we referred to these clusters as the adaptable responsive gene group. These genes were significantly enriched in the ErbB signaling pathway, neuroactive ligand-receptor interaction and type II diabetes mellitus in the KEGG pathways (P < 0.01). From the functional enrichment analysis and significantly regulated genes observed in the enriched pathways, we think that the adaptable responsive genes are responsible for the acclimation mechanism of ducks and suggest that the regulation of phosphoinositide 3-kinase genes including PIK3R6, PIK3R5, and PIK3C2B has an important relationship with the mechanisms of adaptation to heat stress in ducks.
白北京鸭是畜牧业中的一种重要肉用资源。然而，由于全球变暖导致气温升高，已成为鸭生产中的一个严重环境因素，因为高温会导致热应激。因此，鉴定基因调控和了解适应温暖环境的分子机制将为鸭的适应系统提供有见地的信息。本研究使用 RNA 测序分析了来自胸肌组织的基因，以研究热应激处理（35°C 下 3 和 6 小时）和对照（C，25°C）对基因表达的转录组响应。基于三个不同的差异表达基因（DEG）集（3H/C、6H/C 和 6H/3H），对显著 DEGs（绝对对数 2 > 1.0 和错误发现率 < 0.05）的表达模式进行聚类，分为上调和下调基因。接下来，我们分析了在 3H/C 中显示相对较高表达水平而在 6H/C 中表达水平较低、在 6H/3H 中表达水平较低或相反的聚类；我们将这些聚类称为适应性反应基因组。这些基因在 KEGG 通路中的 ErbB 信号通路、神经活性配体-受体相互作用和 II 型糖尿病中显著富集（P < 0.01）。从功能富集分析和富集途径中观察到的显著调节基因来看，我们认为适应性反应基因与鸭的适应机制有关，并表明包括 PIK3R6、PIK3R5 和 PIK3C2B 在内的磷酸肌醇 3-激酶基因的调节与鸭适应热应激的机制密切相关。
