College of Fisheries, Zhejiang Ocean University, Zhoushang 316022, China.
College of Fisheries, Zhejiang Ocean University, Zhoushang 316022, China.
Ecotoxicol Environ Saf. 2024 Sep 15;283:116835. doi: 10.1016/j.ecoenv.2024.116835. Epub 2024 Aug 5.
In recent years, frequent acute temperature changes have posed a serious threat to the physiology and survival of fish. This study utilized RNA-Seq technology to analyze the transcriptional dynamics in the muscle tissues of Acrossocheilus wenchowensis under various acute temperature conditions (16◦C, 20◦C, 24◦C, 28◦C and 32◦C). Through comprehensive analysis, we identified 11509 differentially expressed genes (DEGs), a gene set (profiles 19) that was significantly up-regulated with increasing temperature, and two weighted gene co-expression network analysis (WGCNA) modules that were significantly correlated with acute temperature changes. Furthermore, we identified 28 transcription factors that are pivotal in oxidative stress and energy metabolism under acute temperature changes. Our results showed that, compared to the control group (24°C), KEGG functional enrichment analysis revealed significant enrichment of DEGs in the cell cycle, DNA replication, and p53 signaling pathway, with an overall trend of suppressed expression. This indicates that maintaining cell stability and reducing cell damage is an effective adaptive mechanism for A. wenchowensis to cope with acute temperature changes. Through STEM analysis and the black WGCNA module associated with high-temperature stress, we identified significant up-regulation of pathways and hub genes related to energy metabolism including oxidative phosphorylation, TCA cycle, purine metabolism, and glutathione metabolism, as well as the central roles of signal transduction pathways such as MAPK signaling pathway and AMPK signaling pathway, which synergistically regulate energy production. Under acute low-temperature stress, the turquoise WGCNA module highlighted significant up-regulation of hub genes associated with Ribosomal and Spliceosomal pathways related to protein synthesis and processing, as well as activation of calcium signaling pathways, which plays an important role in maintaining cellular function during low-temperature adaptation. These findings provide a critical theoretical and molecular basis for the adaptation of eurythermal fish to rapid temperature changes.
近年来，频繁的急性温度变化对鱼类的生理和生存造成了严重威胁。本研究利用 RNA-Seq 技术分析了不同急性温度条件（16°C、20°C、24°C、28°C 和 32°C）下圆口铜鱼肌肉组织的转录动态。通过综合分析，我们鉴定了 11509 个差异表达基因（DEGs），一组随温度升高而上调的基因集（profile19），以及两个与急性温度变化显著相关的加权基因共表达网络分析（WGCNA）模块。此外，我们鉴定了 28 个在急性温度变化下对氧化应激和能量代谢至关重要的转录因子。我们的结果表明，与对照组（24°C）相比，KEGG 功能富集分析显示 DEGs 在细胞周期、DNA 复制和 p53 信号通路中显著富集，整体呈下调趋势。这表明维持细胞稳定性和减少细胞损伤是 A. wenchowensis 应对急性温度变化的有效适应机制。通过 STEM 分析和与高温应激相关的黑色 WGCNA 模块，我们鉴定了与氧化磷酸化、TCA 循环、嘌呤代谢和谷胱甘肽代谢等能量代谢相关的途径和枢纽基因显著上调，以及 MAPK 信号通路和 AMPK 信号通路等信号转导途径的核心作用，它们协同调节能量产生。在急性低温应激下，绿松石 WGCNA 模块突出了与核糖体和剪接体途径相关的枢纽基因的显著上调，这些基因与蛋白质合成和加工有关，同时激活钙信号通路，这在低温适应过程中对维持细胞功能起着重要作用。这些发现为广温性鱼类适应快速温度变化提供了重要的理论和分子基础。
