Guo Ying, Wang Zehao, Tian Ye, Li Lin, Dong Jing
College of Animal Science and Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Life (Basel). 2023 Jul 13;13(7):1557. doi: 10.3390/life13071557.
(1) Ferroptosis is a type of cellular death caused by lipid-dependent iron peroxide, which plays a major role in cancer. Long noncoding RNAs (lncRNAs) are increasingly recognized as key regulating substances in ferroptosis; (2) RNA sequencing expressions and clinical data of 519 patients with colon adenocarcinoma (COAD) were downloaded from The Cancer Genome Atlas (TCGA) database. The expression levels of lncRNAs related to ferroptosis were screened with Pearson correlation analysis. Differential genes were enriched with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. LncRNAs related to ferroptosis were determined with univariate Cox regression and multivariate Cox regression analyses, and patients with COAD were classified into high- and low-risk subgroups according to their median risk score. The prognostic value was further examined, and the association between ferroptosis-related lncRNAs (frlncRNAs) and survival in patients with high and low risks of COAD was validated. A TCGA-COAD data set was used for receiver operating characteristic (ROC) analysis and detrended correspondence analysis (DCA) to assess prediction accuracy. Finally, a nomogram was constructed to predict survival probability; (3) We obtained a model consisting of a five-frlncRNAs signature comprising AP003555.1, AP001469.3, ITGB1-DT, AC129492.1, and AC010973.2 for determining the overall survival (OS) of patients with COAD. The survival analysis and ROC curves showed that the model had good robustness and predictive performance on the TCGA training set; (4) We found that a five-frlncRNAs signature may play a potential role in anti-COAD immunity. Risk characteristics based on frlncRNAs can accurately predict the prognosis and immunotherapy response of patients with COAD.
(1) 铁死亡是一种由脂质依赖性过氧化铁引起的细胞死亡类型，在癌症中起主要作用。长链非编码RNA（lncRNA）越来越被认为是铁死亡中的关键调节物质；(2) 从癌症基因组图谱（TCGA）数据库下载了519例结肠腺癌（COAD）患者的RNA测序表达数据和临床数据。通过Pearson相关分析筛选与铁死亡相关的lncRNA表达水平。差异基因通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路进行富集。通过单变量Cox回归和多变量Cox回归分析确定与铁死亡相关的lncRNA，并根据COAD患者的中位风险评分将其分为高风险和低风险亚组。进一步检验预后价值，并验证铁死亡相关lncRNA（frlncRNA）与COAD高风险和低风险患者生存之间的关联。使用TCGA-COAD数据集进行受试者工作特征（ROC）分析和去趋势对应分析（DCA）以评估预测准确性。最后，构建列线图以预测生存概率；(3) 我们获得了一个由五个frlncRNA组成的特征模型，包括AP003555.1、AP001469.3、ITGB1-DT、AC129492.1和AC010973.2，用于确定COAD患者的总生存期（OS）。生存分析和ROC曲线表明该模型在TCGA训练集上具有良好的稳健性和预测性能；(4) 我们发现一个五个frlncRNA的特征可能在抗COAD免疫中发挥潜在作用。基于frlncRNA的风险特征可以准确预测COAD患者的预后和免疫治疗反应。
