Lang Yan, Xu Shuru, Zhang Chunbin
Department of Rehabilitation Therapy, Wuyi University, Nanping 354301, China.
Department of Medical Technology, Zhangzhou Health Vocational College/Collaborative Innovation Center for Translation Medical Testing and Application Technology, Zhangzhou 363000, China.
Micromachines (Basel). 2023 Jul 4;14(7):1368. doi: 10.3390/mi14071368.
In this paper, a molybdenum disulfide fluorescent probe with an Fe fluorescent system was first synthesized by the hydrothermal method for the detection of iron ion concentration in oral solution of protein succinate. It was characterized by infrared, fluorescence, X-ray photoelectron spectroscopy, scanning electron microscopy, and transmission electron microscopy. The probes were found to have good stability, photobleaching, and storage stability. The effects of dilution, pH, reaction time, and iron ion concentration on the fluorescent system were also investigated. The relative fluorescence intensity [(I - I)/I] showed a good linear relationship with the iron ion concentration in the range of 0-50 μM, with the linear equation [(I - I)/I] = 0.0148[Fe] + 0.0833 (r = 0.9943, n = 11) and the detection limit of 2.43 μM. The reaction mechanism was also explored, as well as its ion selectivity, reversibility, accuracy, precision, and concentration of Fe ions in the actual sample. It was found that the probe can selectively detect Fe ions with a certain degree of reversibility, accuracy, precision, and ideal recovery, and it can be used for the determination of Fe in proteosuccinic acid oral solution.
本文首次采用水热法合成了一种具有铁荧光体系的二硫化钼荧光探针，用于检测蛋白琥珀酸口服溶液中的铁离子浓度。通过红外光谱、荧光光谱、X射线光电子能谱、扫描电子显微镜和透射电子显微镜对其进行了表征。结果表明，该探针具有良好的稳定性、抗光漂白性和储存稳定性。研究了稀释、pH值、反应时间和铁离子浓度对荧光体系的影响。相对荧光强度[(I - I)/I]与0-50 μM范围内的铁离子浓度呈现良好的线性关系，线性方程为[(I - I)/I] = 0.0148[Fe] + 0.0833 (r = 0.9943, n = 11)，检测限为2.43 μM。同时探讨了反应机理及其离子选择性、可逆性、准确性、精密度以及实际样品中铁离子的浓度。结果发现，该探针能够选择性地检测铁离子，具有一定程度的可逆性、准确性、精密度和理想的回收率，可用于蛋白琥珀酸口服溶液中铁的测定。
