Fu Tongtong, Fan Zheng, Li Yujie, Li Zhoufei, Zhao Hanqing, Feng Yanling, Xue Guanhua, Cui Jinghua, Yan Chao, Gan Lin, Feng Junxia, Yuan Jing, You Fuping
School of Basic Medical Sciences, Peking University, Beijing 100020, China.
Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China.
Microorganisms. 2023 Jun 25;11(7):1656. doi: 10.3390/microorganisms11071656.
is an opportunistic human pathogen that is often involved in severe infections such as pneumonia and sepsis in which bacterial virulence factors play a key role. Infections caused by are often difficult to eradicate, particularly when they are associated with biofilm. The physiological roles of the Crp/Fnr family regulator ArcR are elusive in . In this study, it was found that the deletion of increased the hemolytic ability and biofilm formation in . Differential gene expression analysis by RNA-seq and real-time quantitative reverse transcription PCR showed that genes associated with hemolytic ability ( and ) and biofilm formation ( and ) were significantly upregulated compared with those in the wild-type strain. The results revealed that ArcR regulated the expression of the and operon by binding to their promoter regions, respectively. This study provided new insights into the functional importance of ArcR in regulating the virulence and biofilm of
是一种机会性人类病原体，常涉及严重感染，如肺炎和败血症，其中细菌毒力因子起关键作用。由 引起的感染往往难以根除，特别是当它们与生物膜相关时。Crp/Fnr家族调节因子ArcR在 中的生理作用尚不清楚。在本研究中，发现缺失 在 中增加了溶血能力和生物膜形成。通过RNA测序和实时定量逆转录PCR进行的差异基因表达分析表明，与溶血能力（ 和 ）和生物膜形成（ 和 ）相关的基因与野生型菌株相比显著上调。结果表明，ArcR分别通过结合其启动子区域来调节 和 操纵子的表达。本研究为ArcR在调节 的毒力和生物膜方面的功能重要性提供了新的见解
