Batoni Giovanna, Kaya Esingül, Catelli Elisa, Quinti Sabrina, Botti Matteo, De Carli Alessandro, Bianchi Marta, Maisetta Giuseppantonio, Esin Semih
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56123 Pisa, Italy.
Cystic Fibrosis Supporting Service, Azienda USL Toscana Nord-Ovest, 57128 Livorno, Italy.
Microorganisms. 2023 Jun 29;11(7):1707. doi: 10.3390/microorganisms11071707.
The field of probiotic applications is rapidly expanding, including their use for the control of respiratory tract infections. Nevertheless, probiotics ability to colonize the lung environment and to compete with pulmonary pathogens is still a poorly investigated research area. In this study, we aimed to evaluate the adhesion ability of a number of commercial probiotic strains to the human lung epithelial cell line A549. Furthermore, we assessed probiotic ability to prevent host cell adhesion of one of the major lung pathogens in cystic fibrosis, , and to reduce the pathogen-induced inflammatory response of human peripheral blood mononuclear cells (PBMCs) in terms of cytokine release. displayed the highest adhesion ability to A549 cells evaluated as percent of adhered bacteria compared to the inoculum. In agreement with such an observation, was the most efficient in preventing adhesion to A549 cells of a isolate from CF sputum. Three-color fluorescence labeling of A549 cells, , and , and confocal microcopy image analyses revealed a likely exclusion effect played by both live and UV-killed towards . Such results were confirmed by CFU count. When co-cultured with PBMCs, both live and UV-killed reduced the amount of IL-1β and IL-6 in culture supernatants in a statistically significant manner. Overall, the results obtained point to as an interesting candidate for further studies for a potential aerogenous administration to control infections.
益生菌应用领域正在迅速扩展，包括其在控制呼吸道感染方面的应用。然而，益生菌在肺部环境中定殖以及与肺部病原体竞争的能力仍是一个研究较少的领域。在本研究中，我们旨在评估多种市售益生菌菌株对人肺上皮细胞系A549的黏附能力。此外，我们评估了益生菌预防囊性纤维化中主要肺部病原体之一黏附宿主细胞的能力，以及就细胞因子释放而言，益生菌降低病原体诱导的人外周血单核细胞（PBMCs）炎症反应的能力。与接种物相比，以黏附细菌百分比评估，[具体菌株1]对A549细胞显示出最高的黏附能力。与这一观察结果一致，[具体菌株1]在预防来自囊性纤维化痰液的[具体病原体]分离株黏附A549细胞方面最为有效。对A549细胞、[具体菌株1]和[具体病原体]进行三色荧光标记以及共聚焦显微镜图像分析显示，活的和经紫外线灭活的[具体菌株1]对[具体病原体]可能发挥了排斥作用。通过菌落形成单位计数证实了这些结果。当与PBMCs共培养时，活的和经紫外线灭活的[具体菌株1]均以统计学显著方式降低了培养上清液中IL-1β和IL-6的量。总体而言，所获得的结果表明[具体菌株1]是一个有趣的候选者，值得进一步研究其通过潜在的气雾剂给药来控制[具体病原体]感染的可能性。
