Department of Antibiotics and Microbiology, National Medicines Institute, Warsaw, Poland.
Department of Pharmaceutical Microbiology, Medical University of Warsaw, Warsaw, Poland.
Arch Microbiol. 2022 Apr 27;204(5):285. doi: 10.1007/s00203-022-02889-8.
The adhesion and aggregation are characteristic attributes of probiotic strains belonging to Lactobacillaceae genus. Due to these properties the host organisms can avoid colonisation of the intestinal tract by enteropathogenic bacteria. The presented research includes a comparison of the properties of various strains belonging to different Lactobacillaceae species and isolated from different sources The aim of this study was to investigate the ability of Lactocaseibacillus rhamnosus, Lactiplantibacillus plantarum, and Lactobacillus strains (L. acidophilus, L. gasseri, L. ultunensis) from probiotic products and clinical specimens to direct and competitive adherence to Caco-2 and HT-29 cell lines. Furthermore, the ability of lactobacilli and enteropathogenic bacteria, E. coli, E. faecalis, and S. Typhimurium, to auto- and co-aggregation was also investigated.The results showed that all tested strains adhered to Caco-2 and HT-29 cell lines. Though, the factor of adhesion depended on the species and origin of the strain. L. rhamnosus strains showed a lowest degree of adherence as compared to L. plantarum and Lactobacillus sp. strains. On the other side both, L. rhamnosus and L. acidophilus strains reduced the pathogenic bacteria in competition adherence test most effectively. All tested lactobacilli strains were characterised by auto- and co-aggregation abilities, to various degrees. The properties of Lactobacillaceae strains analysed in this study, like adhesion abilities, competitive adherence, auto- and co-aggregation, may affect the prevention of colonisation and elimination of pathogenic bacteria in gastrointestinal tract.
黏附和聚集是属于乳杆菌科属的益生菌菌株的特征属性。由于这些特性，宿主生物可以避免肠道被肠致病性细菌定植。本研究包括比较属于不同乳杆菌科物种的不同来源的各种菌株的特性。本研究的目的是研究来自益生菌产品和临床标本的鼠李糖乳杆菌、植物乳杆菌和乳杆菌（嗜酸乳杆菌、格氏乳杆菌、约氏乳杆菌）的直接和竞争黏附能力对 Caco-2 和 HT-29 细胞系。此外，还研究了乳酸菌和肠致病性细菌大肠杆菌、粪肠球菌和鼠伤寒沙门氏菌的自聚集和共聚集能力。结果表明，所有测试的菌株都能黏附到 Caco-2 和 HT-29 细胞系上。然而，黏附因子取决于菌株的种类和来源。与植物乳杆菌和乳杆菌属菌株相比，鼠李糖乳杆菌菌株的黏附程度最低。另一方面，在竞争黏附试验中，无论是鼠李糖乳杆菌还是嗜酸乳杆菌菌株，都能最有效地减少致病菌。所有测试的乳酸菌菌株都具有自聚集和共聚集能力，程度不同。本研究分析的乳杆菌科菌株的特性，如黏附能力、竞争黏附、自聚集和共聚集，可能会影响胃肠道定植和消除致病菌的预防。
