Rocha Rodrigo Theodoro, de Almeida Felipe Marques, Pappas Marília C R, Pappas Georgios Joannis, Martins Karina
Department of Cell Biology, University of Brasília, Brasília 70910-900, DF, Brazil.
EMBRAPA Genetic Resources and Biotechnology, Brasília 70770-917, DF, Brazil.
Microorganisms. 2023 Jun 30;11(7):1729. doi: 10.3390/microorganisms11071729.
The Amazonian rainforest is a hyper-diverse ecosystem in the number of species and the myriad of intertaxon relationships that are mostly understudied. In order to characterize a dominant and economically important Amazonian species, the Brazil nut tree ( Bonpl.), at the genome level, wegenerated high-coverage long-read sequencing data from the leaves of a single individual. The genome assembly revealed an unexpected discovery: two circular contigs that could be assigned to the chromosome and a plasmid of a strain. Comparative genomics revealed that this strain belongs to the subspecies and displays high synteny with other strains isolated from diseased leaves of the neotropical palm Kunth. Investigation of pathogenicity-related genes revealed the absence of the entire type III secretion system gene cluster in the plasmid, which was otherwise highly similar to a plasmid from an isolate known to cause disease in Mast. In contrast, several genes associated with plant-growth promoting traits were detected, including genes involved in indole-3-acetic acid (IAA) production, phosphate solubilization, and biosynthesis of siderophores. In summary, we report the genome of an uncultivated subsp. strain associated with the Brazil nut tree and potentially a plant growth-promoting bacteria.
亚马逊雨林是一个物种数量极其丰富且存在大量尚未得到充分研究的类群间关系的超多样化生态系统。为了在基因组水平上对一种具有主导地位且具有经济重要性的亚马逊物种——巴西坚果（Bertholletia excelsa Bonpl.）进行特征描述，我们从一棵单株的叶片中生成了高覆盖度的长读长测序数据。基因组组装揭示了一个意外发现：两条可被归为一条染色体和一个菌株质粒的环状重叠群。比较基因组学研究表明，该菌株属于[具体亚种名称]亚种，并且与从新热带棕榈（Attalea maripa Kunth）病叶中分离出的其他菌株具有高度的共线性。对致病性相关基因的研究发现，该质粒中不存在整个III型分泌系统基因簇，不过它与一个已知会在[具体植物名称]上致病的分离株的质粒高度相似。相比之下，检测到了几个与促进植物生长特性相关的基因，包括参与吲哚 - 3 - 乙酸（IAA）产生、磷溶解和铁载体生物合成的基因。总之，我们报道了一种与巴西坚果相关的未培养的[具体亚种名称]亚种菌株的基因组，它可能是一种促进植物生长的细菌。
