Botany and Microbiology Department, Faculty of Science, Beni Suef University, Beni Suef 62511, Egypt.
Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, 6720 Szeged, Hungary.
Molecules. 2023 Jul 19;28(14):5501. doi: 10.3390/molecules28145501.
Microalgae have garnered scientific interest for their potential to produce bioactive compounds. However, the large-scale industrial utilization of microalgae faces challenges related to production costs and achieving optimal growth conditions. Thus, this study aimed to investigate the potential role of exogenous indole-3-acetic acid (IAA) application in improving the growth and production of bioactive metabolites in microalgae. To this end, the study employed different concentrations of exogenously administered IAA ranging from 0.36 µM to 5.69 µM to assess its influence on the growth and biochemical composition of and . IAA exposure significantly increased IAA levels in both strains. Consequentially, improved biomass accumulation in parallel with increased total pigment content by approximately eleven-fold in both strains was observed. Furthermore, the application of IAA stimulated the accumulation of primary metabolites. Sugar levels were augmented, providing a carbon source that facilitated amino acid and fatty acid biosynthesis. As a result, amino acid levels were enhanced as well, leading to a 1.55-fold increase in total amino acid content in and a 1.42-fold increase in . Total fatty acids content increased by 1.92-fold in and by 2.16-fold in . Overall, the study demonstrated the effectiveness of exogenously adding IAA as a strategy for enhancing the accumulation of microalgae biomass and biomolecules. These findings contribute to the advancement of microalgae-based technologies, opening new avenues to produce economically important compounds derived from microalgae.
微藻因其具有产生生物活性化合物的潜力而引起了科学界的兴趣。然而，微藻的大规模工业利用面临着与生产成本和实现最佳生长条件相关的挑战。因此，本研究旨在探讨外源性吲哚-3-乙酸（IAA）应用在改善微藻生长和生物活性代谢产物生产中的潜力。为此，该研究采用了不同浓度的外源性 IAA（从 0.36 µM 到 5.69 µM）来评估其对 和 的生长和生化组成的影响。IAA 暴露显著增加了两种菌株的 IAA 水平。因此，观察到两种菌株的生物量积累都有所增加，总色素含量增加了约 11 倍。此外，IAA 的应用刺激了初级代谢物的积累。糖水平增加，提供了碳源，促进了氨基酸和脂肪酸的生物合成。结果，氨基酸水平也得到了提高，导致 和 中的总氨基酸含量分别增加了 1.55 倍和 1.42 倍。和 中的总脂肪酸含量分别增加了 1.92 倍和 2.16 倍。总的来说，该研究表明，外源性添加 IAA 作为一种增强微藻生物量和生物分子积累的策略是有效的。这些发现为基于微藻的技术的发展做出了贡献，为从微藻中生产具有经济重要性的化合物开辟了新的途径。
