Pageaut Héloïse, Lacouture Sonia, Lehoux Mélanie, Marois-Créhan Corinne, Segura Mariela, Gottschalk Marcelo
Swine and Poultry Infectious Diseases Research Center (CRIPA) and Groupe de Recherche sur les Maladies Infectieuses en Production Animale, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte St., Saint-Hyacinthe, QC J2S 2M2, Canada.
Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology Bacteriology and Antimicrobial Resistance Unit, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 22 440 Ploufragan, France.
Pathogens. 2023 Jun 22;12(7):866. doi: 10.3390/pathogens12070866.
Bacterial and/or viral co-infections are very common in swine production and cause severe economic losses. , and are pathogenic bacteria that may be found simultaneously in the respiratory tracts of pigs. In the present study, the interactions of with epithelial and phagocytic cells in the presence or absence of a pre-infection with and/or were studied. Results showed relatively limited interactions between these pathogens. A previous infection with one or both mycoplasmas did not influence the adhesion or invasion properties of in epithelial cells or its resistance to phagocytosis (including intracellular survival) by macrophages and dendritic cells. The most important effect observed during the co-infection was a clear increment in toxicity for the cells. An increase in the relative expression of the pro-inflammatory cytokines IL-6 and CXCL8 was also observed; however, this was the consequence of an additive effect due to the presence of different pathogens rather than a synergic effect. It may be hypothesized that if one or both mycoplasmas are present along with in the lower respiratory tract at the same time, then increased damage to epithelial cells and phagocytes, as well as an increased release of pro-inflammatory cytokines, may eventually enhance the invasive properties of . However, more studies should be carried out to confirm this hypothesis.
细菌和/或病毒混合感染在养猪生产中非常常见，并会造成严重的经济损失。[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]是可能同时存在于猪呼吸道中的致病细菌。在本研究中，研究了在存在或不存在[具体细菌名称1]和/或[具体细菌名称2]预感染的情况下，[具体细菌名称3]与上皮细胞和吞噬细胞之间的相互作用。结果显示这些病原体之间的相互作用相对有限。先前感染一种或两种支原体并不影响[具体细菌名称3]在上皮细胞中的黏附或侵袭特性，也不影响其对巨噬细胞和树突状细胞吞噬作用（包括细胞内存活）的抗性。在混合感染期间观察到的最重要影响是细胞毒性明显增加。还观察到促炎细胞因子IL-6和CXCL8的相对表达增加；然而，这是由于不同病原体的存在产生的累加效应，而非协同效应。可以推测，如果一种或两种支原体与[具体细菌名称3]同时存在于下呼吸道中，那么上皮细胞和吞噬细胞的损伤增加以及促炎细胞因子释放增加，最终可能会增强[具体细菌名称3]的侵袭特性。然而，需要进行更多研究来证实这一假设。
