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斑点叉尾鮰中白细胞介素-10和白细胞介素-6的分子特征及表达分析

Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish ().

作者信息

Zhu Xia, Liu Yongtao, Xu Ning, Ai Xiaohui, Yang Yibin

机构信息

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

出版信息

Pathogens. 2023 Jun 29;12(7):886. doi: 10.3390/pathogens12070886.

DOI:10.3390/pathogens12070886
PMID:37513733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10384647/
Abstract

IL-10 and IL-6 play important roles in protecting against inflammation and clearing pathogens from the body. In this study, homologous compounds of IL-10 and IL-6 were identified in channel catfish, and their immune responses were analyzed. The CDS sequences of IL-10 and IL-6 were 549 bp and 642 bp, respectively, and showed the highest homology with . In addition, the expression of the IL-10 and IL-6 genes was ubiquitous in 10 tissues examined. IL-10 is highly expressed in the liver and slightly expressed in the gill. The high expression of the IL-6 gene was observed in the spleen, heart, and gonad, with the lowest levels in the liver. LPS, Poly(I:C), PHA, and PMA showed a highly significant increase in IL-10 and IL-6 expression 48 h after CCK stimulation ( < 0.01). Otherwise, Yersinia ruckeri, Streptococcus iniae, channel catfish virus, and deltamethrin induced IL-10 and IL-6 expression, varying in intensity between different organs. Our results suggest that IL-10 and IL-6 are involved in the immune response of the host against the pathogen.

摘要

白细胞介素-10（IL-10）和白细胞介素-6（IL-6）在抵御炎症和清除体内病原体方面发挥着重要作用。在本研究中，在斑点叉尾鮰中鉴定出了IL-10和IL-6的同源化合物，并分析了它们的免疫反应。IL-10和IL-6的编码序列（CDS）分别为549 bp和642 bp，与……显示出最高的同源性。此外，IL-10和IL-6基因在检测的10个组织中均普遍表达。IL-10在肝脏中高表达，在鳃中低表达。IL-6基因在脾脏、心脏和性腺中高表达，在肝脏中表达水平最低。脂多糖（LPS）、聚肌苷酸胞苷酸（Poly(I:C)）、植物血凝素（PHA）和佛波酯（PMA）在CCK刺激后48小时显示IL-10和IL-6表达显著增加（<0.01）。此外，鲁氏耶尔森菌、海豚链球菌、斑点叉尾鮰病毒和溴氰菊酯诱导IL-10和IL-6表达，不同器官之间诱导强度有所不同。我们的结果表明，IL-10和IL-6参与宿主针对病原体的免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/8a647a43c2e8/pathogens-12-00886-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/d4306c7c9014/pathogens-12-00886-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/4ac985fa0eb6/pathogens-12-00886-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/1e0e23c8dc7e/pathogens-12-00886-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/80832d35ea6e/pathogens-12-00886-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/7f8149a14eec/pathogens-12-00886-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/9076de8dad9b/pathogens-12-00886-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/8a647a43c2e8/pathogens-12-00886-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/d4306c7c9014/pathogens-12-00886-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/4ac985fa0eb6/pathogens-12-00886-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/1e0e23c8dc7e/pathogens-12-00886-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/80832d35ea6e/pathogens-12-00886-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/7f8149a14eec/pathogens-12-00886-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/9076de8dad9b/pathogens-12-00886-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f2d/10384647/8a647a43c2e8/pathogens-12-00886-g007.jpg

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