Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
The Key Laboratory for Quality and Safety Control of Aquatic Products, Ministry of Agriculture, Beijing, China.
Front Immunol. 2021 Dec 15;12:775708. doi: 10.3389/fimmu.2021.775708. eCollection 2021.
Host-pathogen intectarions are complex, involving large dynamic changes in gene expression through the process of infection. These interactions are essential for understanding anti-infective immunity as well as pathogenesis. In this study, the host-pathogen interaction was analyzed using a model of acute infection where channel catfish were infected with . The infected fish showed signs of body surface hyperemia as well as hyperemia and swelling in the trunk kidney. Double RNA sequencing was performed on trunk kidneys extracted from infected channel catfish and transcriptome data was compared with data from uninfected trunk kidneys. Results revealed that the host-pathogen interaction was dynamically regulated and that the host-pathogen transcriptome fluctuated during infection. More specifically, these data revealed that the expression levels of immune genes involved in Cytokine-cytokine receptor interactions, the NF-kappa B signaling pathway, the JAK-STAT signaling pathway, Toll-like receptor signaling and other immune-related pathways were significantly upregulated. mainly promote pathogenesis through the flagellum gene fli in channel catfish. The weighted gene co-expression network analysis (WGCNA) R package was used to reveal that the infection of catfish is closely related to metabolic pathways. This study contributes to the understanding of the host-pathogen interaction between channel catfish and Y. ruckeri, more specifically how catfish respond to infection through a transcriptional perspective and how this infection leads to enteric red mouth disease (ERM) in these fish.
宿主-病原体相互作用非常复杂，涉及到感染过程中基因表达的大量动态变化。这些相互作用对于理解抗感染免疫和发病机制至关重要。在这项研究中，我们使用急性感染模型分析了宿主-病原体的相互作用，即在感染过程中，斑点叉尾鮰感染了迟缓爱德华氏菌。感染鱼表现出体表充血以及躯干肾脏充血和肿胀的迹象。我们对从感染斑点叉尾鮰中提取的躯干肾脏进行了双链 RNA 测序，并将转录组数据与未感染的躯干肾脏数据进行了比较。结果表明，宿主-病原体的相互作用是动态调节的，宿主-病原体的转录组在感染过程中发生波动。更具体地说，这些数据表明，涉及细胞因子-细胞因子受体相互作用、NF-kappa B 信号通路、JAK-STAT 信号通路、Toll 样受体信号通路和其他免疫相关通路的免疫相关基因的表达水平显著上调。迟缓爱德华氏菌主要通过鞭毛基因 fli 促进致病性。加权基因共表达网络分析（WGCNA）R 包用于揭示鱼类感染与代谢途径密切相关。本研究有助于理解斑点叉尾鮰和迟缓爱德华氏菌之间的宿主-病原体相互作用，更具体地说，斑点叉尾鮰如何通过转录水平来应对感染，以及这种感染如何导致这些鱼类患肠型红嘴病（ERM）。
