Novodiag粪便寄生虫检测法用于检测肠道原生动物和微孢子虫的性能评估。
Evaluation of the Performance of the Novodiag Stool Parasites Assay for the Detection of Intestinal Protozoa and Microsporidia.
作者信息
Chauvin Pamela, Barba Florie, Guemas Emilie, Charpentier Eléna, Cottrel Claire, Fillaux Judith, Valentin Alexis, Baklouti Sarah, Cassaing Sophie, Ménard Sandie, Berry Antoine, Iriart Xavier
机构信息
Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Toulouse, 31059 Toulouse, France.
Institut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), Université de Toulouse, CNRS UMR5051, INSERM UMR1291, Université Paul Sabatier, 31024 Toulouse, France.
出版信息
Pathogens. 2023 Jun 29;12(7):889. doi: 10.3390/pathogens12070889.
OBJECTIVES
We aimed to assess the performance of the Novodiag Stool Parasites (NSP) assay in the diagnosis of the most common intestinal protozoan and microsporidia infections.
METHODS
A panel of 167 selected stool samples was retrospectively analysed with the NSP assay and compared to routine microscopy and qPCR methods for the detection of pathogenic protozoa and microsporidia.
RESULTS
Whereas specificity was high for all protozoa and microsporidia, NSP sensitivity was strongly dependent on the comparative method used as reference. When compared to microscopic methods, NSP sensitivity was high (96.7 to 100%) for , and but was lower for (85.2%) and ≤50% for and . In comparison to conventional qPCR, the NSP assay demonstrated lower sensitivity characteristics dependent on parasite loads, reaching 60 to 70% for , , spp. and Sensitivity was 100% for , but none of the five samples containing spp. were detected.
CONCLUSIONS
The overall performance of the NSP assay in the diagnosis of gastrointestinal protozoa and microsporidia seems to be better than or equivalent to that observed with microscopic methods but inferior to that obtainable with classical targeted qPCR.
目的
我们旨在评估Novodiag粪便寄生虫（NSP）检测法在诊断最常见肠道原生动物和微孢子虫感染方面的性能。
方法
对一组167份选定的粪便样本进行回顾性分析，采用NSP检测法，并与常规显微镜检查和qPCR方法进行比较，以检测致病性原生动物和微孢子虫。
结果
虽然所有原生动物和微孢子虫的特异性都很高，但NSP的敏感性强烈依赖于用作参考的比较方法。与显微镜检查方法相比，NSP对蓝氏贾第鞭毛虫、溶组织内阿米巴和结肠小袋纤毛虫的敏感性较高（96.7%至100%），但对隐孢子虫的敏感性较低（85.2%），对贝氏等孢球虫和微孢子虫的敏感性≤50%。与传统qPCR相比，NSP检测法的敏感性特征较低，取决于寄生虫载量，对微小隐孢子虫、人芽囊原虫、等孢球虫属和环孢子虫的敏感性达到60%至70%。对蓝氏贾第鞭毛虫的敏感性为100%，但未检测到五个含有肠微孢子虫属的样本中的任何一个。
结论
NSP检测法在诊断胃肠道原生动物和微孢子虫方面的总体性能似乎优于或等同于显微镜检查方法，但不如经典靶向qPCR方法。
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