Balcer Emilia, Sobiech Monika, Giebułtowicz Joanna, Sochacka Małgorzata, Luliński Piotr
Department of Drug Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Radiochemistry Team, Reactor Research Division, Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, Świerk, 05-400 Otwock, Poland.
Polymers (Basel). 2023 Jul 24;15(14):3149. doi: 10.3390/polym15143149.
The aim of this study was to create molecularly imprinted polymers (MIPs) that are specific towards 4-borono-L-phenylalanine (BPA) to serve as boron compound carriers. The honeycomb-like MIPs were characterized in the matter of adsorption properties, morphology, structure, and cytotoxicity towards A549 and V79-4 cell lines. The honeycomb-like MIP composed from methacrylic acid and ethylene glycol dimethacrylate was characterized by a binding capacity of 330.4 ± 4.6 ng g and an imprinting factor of 2.04, and its ordered, porous morphology was confirmed with scanning electron microscopy. The theoretical analysis revealed that the coexistence of different anionic forms of the analyte in basic solution might lower the binding capacity of the MIP towards BPA. The release profiles from the model phosphate buffer saline showed that only 0 to 4.81% of BPA was released from the MIP within the time frame of two hours, furthermore, the obtained material was considered non-cytotoxic towards tested cell lines. The results prove that MIPs can be considered as effective BPA delivery systems for biomedical applications and should be investigated in further studies.
本研究的目的是制备对4-硼-L-苯丙氨酸（BPA）具有特异性的分子印迹聚合物（MIP），用作硼化合物载体。对蜂窝状MIP进行了吸附性能、形态、结构以及对A549和V79-4细胞系的细胞毒性等方面的表征。由甲基丙烯酸和乙二醇二甲基丙烯酸酯组成的蜂窝状MIP的结合容量为330.4±4.6 ng/g，印迹因子为2.04，通过扫描电子显微镜确认了其有序的多孔形态。理论分析表明，碱性溶液中分析物不同阴离子形式的共存可能会降低MIP对BPA的结合能力。在模拟磷酸盐缓冲盐水中的释放曲线表明，在两小时的时间范围内，只有0%至4.81%的BPA从MIP中释放出来，此外，所获得的材料对测试细胞系被认为无细胞毒性。结果证明，MIP可被视为生物医学应用中有效的BPA递送系统，应在进一步研究中进行考察。
