一种具有多种酶模拟活性的硝化功能化 MOF，可在中性 pH 下用于比色检测葡萄糖。
A Nitro Functionalized MOF with Multi-Enzyme Mimetic Activities for the Colorimetric Sensing of Glucose at Neutral pH.
机构信息
Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing University of Technology, Chongqing 400050, China.
Chongqing Institute of Innovation and Entrepreneurship for Precision Medicine, Chongqing 400050, China.
出版信息
Sensors (Basel). 2023 Jul 10;23(14):6277. doi: 10.3390/s23146277.
Benefiting from the advantages like large surface area, flexible constitution, and diverse structure, metal-organic frameworks (MOFs) have been one of the most ideal candidates for nanozymes. In this study, a nitro-functionalized MOF, namely NO-MIL-53(Cu), was synthesized. Multi-enzyme mimetic activities were discovered on this MOF, including peroxidase-like, oxidase-like, and laccase-like activity. Compared to the non-functional counterpart (MIL-53(Cu)), NO-MIL-53(Cu) displayed superior enzyme mimetic activities, indicating a positive role of the nitro group in the MOF. Subsequently, the effects of reaction conditions on enzyme mimetic activities were investigated. Remarkably, NO-MIL-53(Cu) exhibited excellent peroxidase-like activity even at neutral pH. Based on this finding, a simple colorimetric sensing platform was developed for the detection of HO and glucose, respectively. The detection liner range for HO is 1-800 μM with a detection limit of 0.69 μM. The detection liner range for glucose is linear range 0.5-300 μM with a detection limit of 2.6 μM. Therefore, this work not only provides an applicable colorimetric platform for glucose detection in a physiological environment, but also offers guidance for the rational design of efficient nanozymes with multi-enzyme mimetic activities.
受益于大表面积、灵活的结构和多样的结构等优点，金属有机骨架（MOFs）已经成为最理想的纳米酶候选物之一。在这项研究中，合成了一种硝基功能化的 MOF，即 NO-MIL-53（Cu）。在这种 MOF 上发现了多种酶模拟活性，包括过氧化物酶样、氧化酶样和漆酶样活性。与非功能化的对应物（MIL-53（Cu））相比，NO-MIL-53（Cu）表现出优越的酶模拟活性，表明 MOF 中的硝基基团发挥了积极作用。随后，研究了反应条件对酶模拟活性的影响。值得注意的是，即使在中性 pH 条件下，NO-MIL-53（Cu）也表现出优异的过氧化物酶样活性。基于这一发现，开发了一种简单的比色传感平台，分别用于检测 HO 和葡萄糖。HO 的检测线性范围为 1-800 μM，检测限为 0.69 μM。葡萄糖的检测线性范围为 0.5-300 μM，检测限为 2.6 μM。因此，这项工作不仅为生理环境中葡萄糖的检测提供了一种可行的比色平台，而且为设计具有多种酶模拟活性的高效纳米酶提供了指导。
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