Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Department of Chemistry, Chung Yuan Christian University, 200 Zhongbei Rd., Zhongli Dist., Taoyuan 320314, Taiwan.
J Mater Chem B. 2023 Nov 8;11(43):10362-10368. doi: 10.1039/d3tb00981e.
Finding materials in biosensing that balance enzyme-like reactivity, stability, and affordability is essential for the future. Because of their unique peroxidase properties, including variable pore size, surface area, and Lewis acid active sites, iron-based metal-organic frameworks (MOFs) have evolved as viable possibilities. In this study, we constructed a Fe-MOF and tested its peroxidase-like activity and responsiveness toward HO colorimetric techniques. Using encapsulation, we incorporated glucose oxidase into the ZIF-90 PVP MOF and conducted a sequential reaction with the Fe-MOF to detect glucose. The results showed better peroxidase catalytic activity of the MIL-88B(Fe) (1,4-NDC) MOF and similar secondary building unit (SBU) Fe-MOFs were studied in other peroxidase nanozyme studies. When combined with an enzyme-encapsulating ZIF-90 PVP MOF, they could be sequentially employed for glucose detection purposes. This study highlights the potential of nanozymes as an alternative to natural enzymes, with promising applications in biosensing and beyond.
在生物传感中寻找平衡酶样反应性、稳定性和可负担性的材料对于未来至关重要。由于其独特的过氧化物酶特性，包括可变孔径、表面积和路易斯酸活性位，基于铁的金属有机骨架（MOFs）已经发展成为可行的选择。在这项研究中，我们构建了一个 Fe-MOF，并测试了其过氧化物酶样活性以及对 HO 比色技术的响应性。我们通过封装将葡萄糖氧化酶纳入 ZIF-90 PVP MOF 中，并与 Fe-MOF 进行连续反应以检测葡萄糖。结果表明，MIL-88B(Fe)（1,4-NDC）MOF 具有更好的过氧化物酶催化活性，而其他过氧化物酶纳米酶研究中也研究了类似的次级结构单元（SBU）Fe-MOF。当与包封酶的 ZIF-90 PVP MOF 结合时，它们可以顺序用于葡萄糖检测。本研究强调了纳米酶作为天然酶替代物的潜力，在生物传感等领域具有广阔的应用前景。
