Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil.
Zoetis Industry of Veterinary Products LTDA, São Paulo 04709-111, Brazil.
Viruses. 2023 Jun 30;15(7):1485. doi: 10.3390/v15071485.
Colibacillosis is a disease caused by and remains a major concern in poultry production, as it leads to significant economic losses due to carcass condemnation and clinical symptoms. The development of antimicrobial resistance is a growing problem of worldwide concern. Lysogenic bacteriophages are effective vectors for acquiring and disseminating antibiotic resistance genes (ARGs). The aim of this study was to investigate the complete genome of isolates from the femurs of Brazilian broiler chickens in order to investigate the presence of antimicrobial resistance genes associated with bacteriophages. Samples were collected between August and November 2021 from broiler batches from six Brazilian states. Through whole genome sequencing (WGS), data obtained were analyzed for the presence of antimicrobial resistance genes. Antimicrobial resistance genes against the aminoglycosides class were detected in 79.36% of the isolates; 74.6% had predicted sulfonamides resistance genes, 63.49% had predicted resistance genes against β-lactams, and 49.2% of the isolates had at least one of the tetracycline resistance genes. Among the detected genes, 27 have been described in previous studies and associated with bacteriophages. The findings of this study highlight the role of bacteriophages in the dissemination of ARGs in the poultry industry.
大肠杆菌病是一种由 引起的疾病，仍然是家禽生产中的主要关注点，因为它会导致由于屠体废弃和临床症状而导致重大经济损失。抗菌药物耐药性的发展是一个日益受到全球关注的问题。溶原性噬菌体是获取和传播抗生素耐药基因（ARGs）的有效载体。本研究的目的是调查从巴西肉鸡股骨中分离的 株的全基因组，以调查与噬菌体相关的抗生素耐药基因的存在。样本于 2021 年 8 月至 11 月间从巴西六个州的肉鸡批次中采集。通过全基因组测序（WGS），分析获得的数据以检测存在的抗生素耐药基因。在 79.36%的分离株中检测到针对氨基糖苷类的抗生素耐药基因；74.6%的分离株预测具有磺胺类耐药基因，63.49%的分离株预测具有针对β-内酰胺类的耐药基因，49.2%的分离株至少具有一种四环素耐药基因。在所检测到的基因中，有 27 个已在先前的研究中描述，并与噬菌体有关。本研究的结果强调了噬菌体在禽类行业中传播 ARGs 的作用。
