Santos Adriele Jéssica do Carmo, Dias Roberto Sousa, da Silva Carlos Henrique Martins, Vidigal Pedro Marcus Pereira, de Sousa Maíra Paula, da Silva Cynthia Canedo, de Paula Sérgio Oliveira
Department of Microbiology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário, 36570-900, Viçosa, Minas Gerais, Brazil.
Department of General Biology, Federal University of Viçosa, Av. Peter Henry Rolfs, s/n, Campus Universitário, 36570-900, Viçosa, Minas Gerais, Brazil.
Access Microbiol. 2024 Aug 15;6(8). doi: 10.1099/acmi.0.000801.v3. eCollection 2024.
Bacteria of the species belong to the family , are Gram-negative bacilli, are moderately thermophilic and are included in the group of thiosulfate-reducing bacteria, being capable of significantly accelerating corrosion in metallic structures. However, no in-depth study on the genome, antibiotic resistance and mobile elements has been carried out so far. In this work, the isolation, phenotypic and genotypic characterization of the multi-resistant UFV_LIMV02 strain was carried out, from water samples from an offshore oil extraction platform in Rio de Janeiro (Brazil). We determined that the isolate has a genome of 2 812 778 bp in size, with 26 % GC content, organized into 34 contigs. Genomic annotation using Rapid Annotation using Subsystem Technology revealed the presence of genes related to resistance to antibiotics and heavy metals. By evaluating the antimicrobial resistance of the isolate using the disc diffusion technique, resistance was verified for the classes of antibiotics, beta-lactams, fluoroquinolones, aminoglycosides, sulfonamides, lincosamides and rifamycins, a total of 14 antibiotics. The search for genomic islands, prophages and defence systems against phage infection revealed the presence of five genomic islands in its genome, containing genes related to resistance to heavy metals and antibiotics, most of which are efflux pumps and several transposases. No prophage was found in its genome; however, nine different defence systems against phage infection were detected. When analysing the clustered regularly interspaced short palindromic repeat (CRISPR) systems, four CRISPR arrays, classified as types I-B and III-B, with 272 spacers, can provide the strain with immunity to different mobile genetic elements and bacteriophage infection. The results found in this study show that the isolate UFV_LIVM02 is an environmental bacterium, resistant to different classes of antibiotics, and that the proteins encoded by the predicted genomic islands may be associated with the development of greater resistance to antibiotics and heavy metals. They provide evidence that environmental bacteria found in offshore oil exploration residues may pose a risk for the spread of antibiotic resistance genes. More comprehensive studies on the microbial community present in oil waste are needed to assess the risks of horizontal gene transfer.
该物种的细菌属于 科，为革兰氏阴性杆菌，具有中度嗜热性，属于硫代硫酸盐还原细菌群，能够显著加速金属结构的腐蚀。然而，迄今为止尚未对其基因组、抗生素抗性和移动元件进行深入研究。在这项工作中，从巴西里约热内卢一个海上石油开采平台的水样中分离出多重耐药的UFV_LIMV02菌株，并对其进行了表型和基因型特征分析。我们确定该分离株的基因组大小为2812778 bp，GC含量为26%，由34个重叠群组成。使用子系统技术快速注释进行的基因组注释揭示了与抗生素和重金属抗性相关的基因的存在。通过使用纸片扩散技术评估该分离株的抗菌抗性，验证了其对β-内酰胺类、氟喹诺酮类、氨基糖苷类、磺胺类、林可酰胺类和利福霉素类抗生素的抗性，总共14种抗生素。对基因组岛、原噬菌体和针对噬菌体感染的防御系统的搜索揭示了其基因组中存在五个基因组岛，包含与重金属和抗生素抗性相关的基因，其中大多数是外排泵和几种转座酶。在其基因组中未发现原噬菌体；然而，检测到九种不同的针对噬菌体感染的防御系统。在分析成簇规律间隔短回文重复序列（CRISPR）系统时，四个CRISPR阵列，分类为I-B型和III-B型，有272个间隔序列，可以为该菌株提供对不同移动遗传元件和噬菌体感染的免疫力。本研究中发现的结果表明，分离株UFV_LIVM02是一种环境细菌，对不同类别的抗生素具有抗性，并且预测的基因组岛编码的蛋白质可能与对抗生素和重金属的更强抗性的发展有关。它们提供了证据表明在海上石油勘探残渣中发现的环境细菌可能对抗生素抗性基因的传播构成风险。需要对油废物中存在的微生物群落进行更全面的研究，以评估水平基因转移的风险。
