Quality Assurance Department, Bio-Manguinhos-Fiocruz, Rio de Janeiro 21040-900, Brazil.
Vice Director of Quality, Bio-Manguinhos-Fiocruz, Rio de Janeiro 21040-900, Brazil.
Viruses. 2023 Jul 23;15(7):1609. doi: 10.3390/v15071609.
The aim of this study is to evaluate some mechanisms of the immune response of people infected with SARS-CoV-2 in both acute infection and early and late convalescence phases.
This is a cohort study of 70 cases of COVID-19, confirmed by RT-PCR, followed up to 60 days. Plasma Samples and clinical data were. Viral load, blood count, indicators inflammation were the parameters evaluated. Cellular immune response was evaluated by flow cytometry and Luminex immunoassays.
In the severe group, hypertension was the only reported comorbidity. Non severe patients have activated memory naive CD4+ T cells. Critically ill patients have central memory CD4+ T cell activation. Severe COVID-19 patients have both central memory and activated effector CD8+ T cells. Non-severe COVID-19 cases showed an increase in IL1β, IL-6, IL-10 and TNF and severely ill patients had higher levels of the cytokines IL-6, IL-10 and CXCL8.
The present work showed that different cellular responses are observed according to the COVID-19 severity in patients from Brazil an epicenter the pandemic in South America. Also, we notice that some cytokines can be used as predictive markers for the disease outcome, possibility implementation of strategies effective by health managers.
本研究旨在评估 SARS-CoV-2 感染患者在急性感染期、早期和晚期恢复期的免疫反应的一些机制。
这是一项队列研究，共纳入 70 例经 RT-PCR 确诊的 COVID-19 患者，随访时间为 60 天。评估了病毒载量、血常规、炎症指标等参数。采用流式细胞术和 Luminex 免疫分析评估细胞免疫反应。
在重症组中，高血压是唯一报告的合并症。非重症患者有激活的记忆性幼稚 CD4+T 细胞。危重症患者有中央记忆性 CD4+T 细胞激活。重症 COVID-19 患者既有中央记忆性和激活效应性 CD8+T 细胞。非重症 COVID-19 病例显示 IL1β、IL-6、IL-10 和 TNF 增加，重症患者的 IL-6、IL-10 和 CXCL8 细胞因子水平更高。
本研究表明，根据巴西（南美洲大流行的中心）患者 COVID-19 的严重程度，观察到不同的细胞反应。此外，我们注意到一些细胞因子可以作为疾病结局的预测标志物，为卫生管理者实施有效的策略提供了可能。
