Guo Hui, Zang Lichao, Yang Xinyu, Wu Yumin, Ma Jinhong, Shi Weifeng
Department of Laboratory Medicine, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
Department of Laboratory Medicine, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Aug;39(8):729-736.
Objectives Objectives To investigate how the imbalance of innate lymphoid cells (ILCs)in the peripheral blood of patients with lung adenocarcinoma affects the balance of downstream mononuclear macrophages and T helper (Th) cells, and to identify the impact of the imbalance of ILCs on the immune status and prognosis of lung adenocarcinoma. Methods The peripheral blood of 20 patients with lung adenocarcinoma and normal controls were collected. The percentage of ILCs, mononuclear macrophages and T lymphocyte in peripheral blood were analyzed by flow cytometry. The characteristic cytokine secretion levels of various types of immune cells in peripheral blood were detected by real-time fluorescence quantitative PCR. Results Compared with the normal controls, the proportion of M2 mononuclear macrophages, ILC1 and ILC2 in patients with lung adenocarcinoma was up-regulated, while the proportion of M1 mononuclear macrophages, CD4 T and CD8 T was down-regulated. The mRNA expression of related cytokines of M1 mononuclear macrophages and ILC1 were decreased; while the mRNA expression of related cytokines of M2 mononuclear macrophages and ILC2 were increased. Along with the decreased CD4T cells-associated cytokine T-bet mRNA expression, and the increased GATA3 mRNA expression. Moreover, the expression of PD-1 in CD8 T cells was also up-regulated. Conclusion The imbalance of ILCs in peripheral blood of patients with lung adenocarcinoma promotes the imbalance of mononuclear macrophages and Th cells, which altogether maintains the immunosuppression in patients with lung adenocarcinoma, and promotes the development of lung adenocarcinoma.
目的 研究肺腺癌患者外周血中固有淋巴细胞（ILC）失衡如何影响下游单核巨噬细胞和辅助性T（Th）细胞平衡，明确ILC失衡对肺腺癌免疫状态及预后的影响。方法 收集20例肺腺癌患者及正常对照者的外周血。采用流式细胞术分析外周血中ILC、单核巨噬细胞及T淋巴细胞的百分比。采用实时荧光定量PCR检测外周血中各类免疫细胞特征性细胞因子分泌水平。结果 与正常对照相比，肺腺癌患者M2单核巨噬细胞、ILC1和ILC2比例上调，而M1单核巨噬细胞、CD4 T和CD8 T比例下调。M1单核巨噬细胞和ILC1相关细胞因子的mRNA表达降低；而M2单核巨噬细胞和ILC2相关细胞因子的mRNA表达增加。同时，CD4T细胞相关细胞因子T-bet mRNA表达降低，GATA3 mRNA表达增加。此外，CD8 T细胞中PD-1的表达也上调。结论 肺腺癌患者外周血ILC失衡促进单核巨噬细胞和Th细胞失衡，共同维持肺腺癌患者的免疫抑制状态，促进肺腺癌进展。
