Kyoto Pharmaceutical University, Kyoto City, Kyoto Prefecture 607-8414, Japan.
Institute for Protein Research, Osaka University, Suita City, Osaka Prefecture 565-0871, Japan.
STAR Protoc. 2023 Sep 15;4(3):102454. doi: 10.1016/j.xpro.2023.102454. Epub 2023 Jul 28.
In membrane proteins, a transmembrane region and a juxtamembrane region play important roles in its function. Here, we present a protocol for characterizing membrane protein dynamics between the juxtamembrane region of the single transmembrane protein and acidic membrane. We describe steps for solid-phase peptide synthesis, peptide purification, and labeling. We then detail reconstitution of the transmembrane peptide into lipid bilayers and its evaluation and structural analysis. For complete details on the use and execution of this protocol, please refer to Prasada Rao et al..
在膜蛋白中，跨膜区域和近膜区域在其功能中起着重要作用。在这里，我们提出了一种用于研究单跨膜蛋白的近膜区域与酸性膜之间的膜蛋白动力学的方案。我们描述了固相肽合成、肽纯化和标记的步骤。然后详细介绍了跨膜肽在脂质双层中的重建及其评估和结构分析。有关此方案使用和执行的完整详细信息，请参阅 Prasada Rao 等人的研究。
