人类诱导多能干细胞源性多巴胺能神经元钙调控失调测量方案。

Neuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

STAR Protoc. 2021 Mar 23;2(2):100405. doi: 10.1016/j.xpro.2021.100405. eCollection 2021 Jun 18.

Calcium regulation is a critical process in neurons, and Ca signaling is a major contributor to neurological disorders including Parkinson's disease (PD). Here, combining calcium imaging with whole-cell Ca current recording, we provide a detailed protocol for measuring Ca homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

钙调节是神经元中的一个关键过程，钙信号是包括帕金森病（PD）在内的神经紊乱的主要诱因。在这里，我们结合钙成像和全细胞膜片钳钙电流记录，提供了一个详细的方案，用于测量源自人诱导多能干细胞（hiPSC）的多巴胺能（DA）神经元中的钙动态平衡。这种方法可用于研究钙动态平衡在神经元功能以及疾病过程中的作用。有关该方案使用和实施的完整详细信息，请参考 Kim 等人（2020 年）。