Shivaram Karthik Basthi, Bhatt Pankaj, Verma Mohit S, Clase Kari, Simsek Halis
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47906, USA.
Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47906, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47906, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA.
Sci Total Environ. 2023 Nov 25;901:165859. doi: 10.1016/j.scitotenv.2023.165859. Epub 2023 Jul 27.
Wastewater is discarded from several sources, including industry, livestock, fertilizer application, and municipal waste. If the disposed of wastewater has not been treated and processed before discharge to the environment, pathogenic microorganisms and toxic chemicals are accumulated in the disposal area and transported into the surface waters. The presence of harmful microbes is responsible for thousands of human deaths related to water-born contamination every year. To be able to take the necessary step and quick action against the possible presence of harmful microorganisms and substances, there is a need to improve the effective speed of identification and treatment of these problems. Biosensors are such devices that can give quantitative information within a short period of time. There have been several biosensors developed to measure certain parameters and microorganisms. The discovered biosensors can be utilized for the detection of axenic and mixed microbial strains from the wastewaters. Biosensors can further be developed for specific conditions and environments with an in-depth understanding of microbial organization and interaction within that community. In this regard, bacteriophage-based biosensors have become a possibility to identify specific live bacteria in an infected environment. This paper has investigated the current scenario of microbial community analysis and biosensor development in identifying the presence of pathogenic microorganisms.
废水来自多个源头，包括工业、畜牧业、肥料施用和城市垃圾。如果未经处理和加工就将废水排放到环境中，致病微生物和有毒化学物质就会在排放区域积累，并进入地表水。有害微生物的存在每年导致数千人死于与水传播污染相关的疾病。为了能够针对有害微生物和物质的可能存在采取必要措施并迅速行动，需要提高识别和处理这些问题的有效速度。生物传感器就是这样一种能够在短时间内给出定量信息的设备。已经开发出了几种用于测量某些参数和微生物的生物传感器。已发现的生物传感器可用于检测废水中的无菌和混合微生物菌株。通过深入了解特定环境中微生物群落的组织和相互作用，还可以进一步开发适用于特定条件和环境的生物传感器。在这方面，基于噬菌体的生物传感器已成为在感染环境中识别特定活细菌的一种可能。本文研究了微生物群落分析和生物传感器开发在识别致病微生物存在方面的现状。
