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利用仿生肽和噬菌体探针在电化学生物传感中突破障碍。

Breaking barriers in electrochemical biosensing using bioinspired peptide and phage probes.

机构信息

Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Pza. de las Ciencias 2, Madrid, 28040, Spain.

Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, Majadahonda, Madrid, 28220, Spain.

出版信息

Anal Bioanal Chem. 2024 Dec;416(30):7225-7247. doi: 10.1007/s00216-024-05294-w. Epub 2024 Apr 19.

DOI:10.1007/s00216-024-05294-w
PMID:38639792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11584481/
Abstract

Electrochemical biosensing continues to advance tirelessly, overcoming barriers that have kept it from leaving research laboratories for many years. Among them, its compromised performance in complex biological matrices due to fouling or receptor stability issues, the limitations in determining toxic and small analytes, and its use, conditioned to the commercial availability of commercial receptors and the exploration of natural molecular interactions, deserved to be highlighted. To address these challenges, in addition to the intrinsic properties of electrochemical biosensing, its coupling with biomimetic materials has played a fundamental role, among which bioinspired phage and peptide probes stand out. The versatility in design and employment of these probes has opened an unimaginable plethora of possibilities for electrochemical biosensing, improving their performance far beyond the development of highly sensitive and selective devices. The state of the art offers robust electroanalytical biotools, capable of operating in complex samples and with exciting opportunities to discover and determine targets regardless of their toxicity and size, the commercial availability of bioreceptors, and prior knowledge of molecular interactions. With all this in mind, this review offers a panoramic, novel, and updated vision of both the tremendous advances and opportunities offered by the combination of electrochemical biosensors with bioinspired phage and peptide probes and the challenges and research efforts that are envisioned in the immediate future.

摘要

电化学生物传感技术不断不懈地进步，克服了多年来使其无法离开研究实验室的障碍。其中，由于污染或受体稳定性问题，它在复杂生物基质中的性能受到限制，对有毒和小分析物的测定受到限制，以及其使用受到限制，这取决于商业上可用的商业受体和对天然分子相互作用的探索，值得强调。为了解决这些挑战，除了电化学生物传感的固有特性外，它与仿生材料的结合也起到了至关重要的作用，其中受生物启发的噬菌体和肽探针尤为突出。这些探针在设计和应用上的多功能性为电化学生物传感开辟了难以想象的可能性，极大地提高了其性能，远远超出了开发高灵敏度和选择性器件的范畴。最先进的技术提供了强大的电分析生物工具，能够在复杂的样品中运行，并提供了令人兴奋的机会，可以发现和确定目标，无论其毒性和大小、生物受体的商业可用性以及分子相互作用的先验知识如何。考虑到这一切，这篇综述提供了一个全景式的、新颖的和更新的视角，展示了电化学生物传感器与受生物启发的噬菌体和肽探针结合所带来的巨大进展和机遇，以及在不久的将来所面临的挑战和研究工作。

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