Iwamaru Yoshifumi, Furusaki Koichi, Sugiura Katsuaki, Haritani Makoto, Onodera Takashi
National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Mineral Activation Technical Research Center, Omuta, Fukuoka, Japan.
Microbiol Immunol. 2023 Oct;67(10):447-455. doi: 10.1111/1348-0421.13092. Epub 2023 Jul 30.
Prion diseases are fatal neurodegenerative disorders affecting both humans and animals. The causative agent, prion, is extremely resistant to common disinfection procedures. Thus, effective prion inactivation strategies using relatively safe and less corrosive disinfectants are required. A solution containing CAC-717, mesoscopic crystals of calcium bicarbonate, exerts both antimicrobial and virucidal activities without apparent harmful effects. This study demonstrated that combined treatment with CAC-717 absorbed on ceramic (CAC-717 ceramic) and sodium dodecyl sulfate (SDS) substantially reduced the protein misfolding cyclic amplification (PMCA) seeding activity of Chandler strain scrapie mouse-brain homogenates (ScBH). Additionally, bioassays demonstrated that ScBH-inoculated mice treated with CAC-717 ceramic in combination with sodium dodecyl sulfate (SDS) did not develop disease. Furthermore, this combination effectively inactivated PMCA seeding activity on ScBH-coated stainless-steel wires below the detection limit. Overall, the findings suggest that combined treatment with CAC-717 ceramic and SDS represents a promising and less damaging approach for prion inactivation.
朊病毒疾病是影响人类和动物的致命性神经退行性疾病。其病原体朊病毒对常见的消毒程序具有极强的抵抗力。因此，需要采用相对安全且腐蚀性较小的消毒剂来制定有效的朊病毒灭活策略。一种含有CAC - 717（碳酸氢钙介观晶体）的溶液具有抗菌和杀病毒活性，且无明显有害影响。本研究表明，用吸附在陶瓷上的CAC - 717（CAC - 717陶瓷）与十二烷基硫酸钠（SDS）联合处理，可显著降低钱德勒毒株羊瘙痒病小鼠脑匀浆（ScBH）的蛋白质错误折叠循环扩增（PMCA）种子活性。此外，生物测定表明，用CAC - 717陶瓷与十二烷基硫酸钠（SDS）联合处理接种ScBH的小鼠未发病。此外，这种组合能有效将ScBH包被的不锈钢丝上的PMCA种子活性灭活至检测限以下。总体而言，研究结果表明，CAC - 717陶瓷与SDS联合处理是一种有前景且破坏性较小的朊病毒灭活方法。
