Della Salda Leonardo, Bongiovanni Laura, Massimini Marcella, Romanucci Mariarita, Vercelli Antonella, Colosimo Alessia, Di Matteo Ramona, Defourny Sabrina Vanessa Patrizia
Department of Veterinary Medicine, University of Teramo, Teramo, Italy.
Department of Biomolecular Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Vet Dermatol. 2023 Dec;34(6):567-575. doi: 10.1111/vde.13195. Epub 2023 Jul 30.
Alopecia X in Pomeranians is caused by a hair cycle deregulation, associated with downregulation of key regulatory genes of the Wnt and Shh pathways, and stem-cell markers. However, the pathogenesis remains unclear. p63 is an important transcription factor correlated with the aforementioned hair cycle modulating genes.
HYPOTHESIS/OBJECTIVES: The aim of this study was to highlight possible changes of p63 immunohistochemical expression within the hair follicles in canine alopecia X compared with normal skin.
Skin biopsies from 19 alopecia X-affected and six control Pomeranians were analysed.
Serial histological sections of skin biopsies harbouring anagen, telogen and kenogen hair follicles were immunohistochemically evaluated for differences in p63 expression in the affected and control samples.
Dogs with alopecia X had a significantly decreased immunoexpression of p63 in telogen and kenogen hair follicles.
The decrease of p63 immunoexpression observed in canine alopecia X suggests an involvement of p63 in hair cycle.
博美犬的X型脱发是由毛囊周期失调引起的，与Wnt和Shh信号通路的关键调控基因以及干细胞标志物的下调有关。然而，其发病机制仍不清楚。p63是一种与上述毛囊周期调节基因相关的重要转录因子。
假设/目的：本研究的目的是突出与正常皮肤相比，犬X型脱发毛囊中p63免疫组化表达的可能变化。
分析了19只患X型脱发的博美犬和6只对照博美犬的皮肤活检样本。
对含有生长期、休止期和退行期毛囊的皮肤活检样本的连续组织切片进行免疫组化评估，以检测患病样本和对照样本中p63表达的差异。
患X型脱发的犬在休止期和退行期毛囊中p63的免疫表达显著降低。
在犬X型脱发中观察到的p63免疫表达降低表明p63参与了毛囊周期。
