Bhattacharjee Abhishek, Sabino Roberta M, Gangwish Justin, Manivasagam Vignesh K, James Susan, Popat Ketul C, Reynolds Melissa, Li Yan Vivian
School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO USA.
Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
In Vitro Model. 2022;1(3):241-247. doi: 10.1007/s44164-022-00022-z. Epub 2022 Jun 1.
SARS-CoV-2 is a pandemic coronavirus that causes severe respiratory disease (COVID-19) in humans and is responsible for millions of deaths around the world since early 2020. The virus affects the human respiratory cells through its spike (S) proteins located at the outer shell. To monitor the rapid spreading of SARS-CoV-2 and to reduce the deaths from the COVID-19, early detection of SARS-CoV-2 is of utmost necessity. This report describes a flexible colorimetric biosensor capable of detecting the S protein of SARS-CoV-2. The colorimetric biosensor is made of polyurethane (PU)-polydiacetylene (PDA) nanofiber composite that was chemically functionalized to create a binding site for the receptor molecule-nucleocapsid antibody (anti-N) protein of SARS-CoV-2. After the anti-N protein conjugation to the functionalized PDA fibers, the PU-PDA-NHS-anti fiber was able to detect the S protein of SARS-CoV-2 at room temperature via a colorimetric transition from blue to red. The PU-PDA nanofiber-based biosensors are flexible and lightweight and do not require a power supply such as a battery when the colorimetric detection to S protein occurs, suggesting a sensing platform of wearable devices and personal protective equipment such as face masks and medical gowns for real-time monitoring of virus contraction and contamination. The wearable biosensors could significantly power mass surveillance technologies to fight against the COVID-19 pandemic.
The online version contains supplementary material available at 10.1007/s44164-022-00022-z.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种大流行性冠状病毒，可导致人类患上严重呼吸道疾病（COVID-19），自2020年初以来已在全球造成数百万人死亡。该病毒通过其位于外壳的刺突（S）蛋白影响人类呼吸道细胞。为监测SARS-CoV-2的快速传播并减少COVID-19导致的死亡，尽早检测SARS-CoV-2至关重要。本报告描述了一种能够检测SARS-CoV-2 S蛋白的柔性比色生物传感器。该比色生物传感器由聚氨酯（PU）-聚二乙炔（PDA）纳米纤维复合材料制成，经过化学功能化处理以创建与SARS-CoV-2受体分子核衣壳抗体（抗N）蛋白的结合位点。在抗N蛋白与功能化的PDA纤维结合后，PU-PDA-NHS-抗纤维能够在室温下通过从蓝色到红色的比色转变检测SARS-CoV-2的S蛋白。基于PU-PDA纳米纤维的生物传感器具有柔性且重量轻，在对比色检测S蛋白时不需要电池等电源，这表明其可作为可穿戴设备以及口罩和医用防护服等个人防护装备的传感平台，用于实时监测病毒感染和污染情况。这种可穿戴生物传感器可为大规模监测技术提供强大助力，以对抗COVID-19大流行。
在线版本包含可在10.1007/s44164-022-00022-z获取的补充材料。
