Zhang Qin, Feng Rencai, Miao Renyun, Lin Junbin, Cao Luping, Ni Yanqing, Li Wensheng, Zhao Xu
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, 610299, Sichuan, China.
Chengdu National Agricultural Science and Technology Center, Chengdu, 610299, Sichuan, China.
Heliyon. 2023 Jul 17;9(7):e18360. doi: 10.1016/j.heliyon.2023.e18360. eCollection 2023 Jul.
, one of the most highly regarded edible mushrooms in China, is susceptible to damage from high temperatures. However, a mutant strain derived from , known as Le023M, has shown exceptional thermotolerance. Compared to the original strain Le023, Le023M exhibited accelerated mycelial recovery following heat stress. Through RNA-seq analysis, the majority of differentially expressed genes (DEGs) were found to be associated with functions such as "protein refolding", "protein unfolding", "protein folding", and "response to heat", all of which are closely linked to heat shock proteins. Furthermore, qRT-PCR results revealed significant accumulation of heat shock-related genes in Le023M under heat stress. GC-MS analysis indicated elevated levels of trehalose, aspartate, and glutamate in Le023M when subjected to heat stress. The highly expressed genes involved in these metabolic pathways were predominantly found in Le023M. Collectively, these findings highlight the following: (i) the crucial role of heat shock proteins (HSPs) in the thermo-resistant mechanisms of Le023M; (ii) the potential of trehalose accumulation in Le023M to enhance mycelium resistance to heat stress; and (iii) the induction of aspartate and glutamate accumulation in response to heat stress. These results shed light on the molecular mechanisms underlying the thermotolerance of Le023M, providing valuable insights for further understanding and improving heat stress response in . The findings also highlight the potential applications of Le023M in the cultivation and production of under high-temperature conditions.
在中国，[某种蘑菇名称未给出]是最受推崇的可食用蘑菇之一，但易受高温损害。然而，从[某种蘑菇名称未给出]衍生出的一个突变菌株Le023M表现出了卓越的耐热性。与原始菌株Le023相比，Le023M在热应激后菌丝体恢复加快。通过RNA测序分析发现，大多数差异表达基因（DEGs）与“蛋白质重折叠”、“蛋白质解折叠”、“蛋白质折叠”和“热响应”等功能相关，所有这些都与热休克蛋白密切相关。此外，qRT-PCR结果显示，在热应激下Le023M中热休克相关基因大量积累。气相色谱-质谱联用（GC-MS）分析表明，Le023M在热应激时海藻糖、天冬氨酸和谷氨酸水平升高。参与这些代谢途径的高表达基因主要在Le023M中被发现。总体而言，这些发现突出了以下几点：（i）热休克蛋白（HSPs）在Le023M耐热机制中的关键作用；（ii）Le023M中海藻糖积累增强菌丝体耐热应激的潜力；（iii）热应激诱导天冬氨酸和谷氨酸积累。这些结果揭示了Le023M耐热性的分子机制，为进一步理解和改善[某种蘑菇名称未给出]的热应激反应提供了有价值的见解。这些发现还突出了Le023M在高温条件下[某种蘑菇名称未给出]栽培和生产中的潜在应用。
